Ain Shams Scholar Collection:
http://hdl.handle.net/123456789/633
2024-03-29T15:51:50ZBiosynthesis and biocompatibility evaluation of zinc oxide nanoparticles prepared using Priestia megaterium bacteria
http://hdl.handle.net/123456789/213954
Title: Biosynthesis and biocompatibility evaluation of zinc oxide nanoparticles prepared using Priestia megaterium bacteria
Authors: Ashour, Mona A.; Abd-Elhalim, Basma T.2024-01-01T00:00:00ZEffect of extenders, cryoprotectants, and freezing methods on sperm quality and fertilisation rate of the European sea bass (Dicentrarchus Labrax)
http://hdl.handle.net/123456789/213941
Title: Effect of extenders, cryoprotectants, and freezing methods on sperm quality and fertilisation rate of the European sea bass (Dicentrarchus Labrax)
Authors: Badr, Magdy R.; Rawash, Zaher M.; Hassan, Hany M.; Elaraby, Mohamed E.; Amer, Mohamed A.; Kareem M. Ahmed
Abstract: Fish semen cryopreservation is a valuable technique to sustain good quality sperm for fertilization, facilitating threatened species conservation and broodstock genetic development. The present investigation aimed to develop suitable standardized protocols to cryopreserve sea bass (Dicentrarchus labrax) semen. Thus, four experiments were conducted to determine the most effective extender (egg-yolk citrate, urea-egg-yolk, and 0.9% NaCl), cryoprotectant (DMSO, glycerol, ethylene glycol “EG”, and methanol), sperm/cryodiluent dilution ratio (1:2, 1:4, 1:10, and 1:20) and equilibration time (0, 5, 15, or 30 min) to maintain the sperm content after cryopreservation. The consistency of post-thaw semen was assessed, with motility percentage, motility duration, and fertilization rate all being taken into account. When sperms were thawed, the consistency was at its best, especially when sperm was diluted with a ratio of 1:20 with egg-yolk citrate extender containing 10% DMSO, with a 15-min equilibration period before cryopreservation. Sea bass sperm cryopreserved using this protocol exhibited fertilisation rates, class motility, and motility duration close to fresh semen. Thus, the cryopreservation method developed in the present investigation proved to be an efficient and effective approach to augment the cryosurvival of sea bass spermatozoa.2021-01-01T00:00:00ZRole of Vitamin C Supplementation in Alleviation of Aflatoxin- Contaminated Feed of Nile tilapia (Oreochromis niloticus)
http://hdl.handle.net/123456789/213940
Title: Role of Vitamin C Supplementation in Alleviation of Aflatoxin- Contaminated Feed of Nile tilapia (Oreochromis niloticus)
Authors: El-Nahal, Sara; Mohamed A. Amer; Mohamed F. Osman; Kareem M. Ahmed
Abstract: The purpose of the research was to illustrate the effects of feeding Nile tilapia fingerlings (Oreochromis niloticus) with low doses of aflatoxins (AFs) with and without vitamin C supplementation to investigate the capac-ity of vitamin C in detoxification. Ten experimental diets were formulated and divided into three categories. The first category included: T1, T4 and T7 feedings included 20, 40 and 80 μg AFs kg−1 feed respectively, while both the second category T2, T5 and T8 and the third category T3, T6 and T9 were treated with contaminated feed with AFs for 57days then both catego-ries shifted to different regime till the conclusion of the experimentation. The second category was fed uncontaminated feed while the third category was fed a supplemented diet with 100 mg Kg-1 of vitamin C. For 113 days the experiment was conducted. The results showed that shifting from a con-taminated diet to an uncontaminated diet (category 2) or adding vitamin C to the contaminated diet (category 3) improved the deterioration that oc-curred in the values of growth performance, biochemical parameters and his-tological disorders caused by AFs. Furthermore, the results from the control group were superior to all the treatments.2023-12-01T00:00:00ZEffect of Substituting Soybean Meal in Oreochromis niloticus Diets with Pumpkin (Cucurbita maxima) Seed Cake on Water Quality, Growth, Antioxidant Capacity, Immunity, and Carcass Composition
http://hdl.handle.net/123456789/213939
Title: Effect of Substituting Soybean Meal in Oreochromis niloticus Diets with Pumpkin (Cucurbita maxima) Seed Cake on Water Quality, Growth, Antioxidant Capacity, Immunity, and Carcass Composition
Authors: Mounes, Hossam A M; Abd-El Azeem, Zeinab M A; Abd El-Bary, Dena A; Al-Sagheer, Adham A; Abd-Elhakim, Yasmina M; Hassan, Bayan A; Sadek, Sherif S; Kareem M. Ahmed
Abstract: A 10-week feeding experiment was performed to determine the impacts of partial substitution of soybean meal (SB) with pumpkin seed cake (PSC) in Oreochromis niloticus diets on water quality, growth rate, antioxidant capacity, immunity, and carcass composition. One hundred and fifty tilapia fish (average weight, 11.93 ± 0.17 g) were randomly allocated to five diets. The first diet (the basal diet) contained 420 g of SB per kg of feed. The remaining four diets, namely, D1, D2, D3, and D4, had SB partially replaced by PSC at 10%, 20%, 30%, and 40%, respectively. The results revealed that D4 and D1 significantly improved dissolved oxygen levels, while water temperature, pH, total ammonia, and nitrate levels were not significantly affected. Replacing SB with PSC significantly improved specific growth performance indicators and feed conversion compared to the control, with the D4 group showing the best values. Increasing PSC levels decreased serum glucose, aspartate aminotransferase, alanine aminotransferase, cholesterol, and triglyceride levels. In contrast, the D4 group had higher globulin, albumin, total protein, and lysozyme serum levels. Moreover, fish-fed PSC had significantly increased superoxide dismutase, glutathione peroxidase, and catalase activities and significantly decreased malondialdehyde levels. Increasing PSC substitution levels in fish diets increased the ash and crude lipid contents in the bodies of the fish, while crude protein and moisture decreased. In conclusion, replacing SB with PSC in fish diets significantly enhances growth performance, feed conversion, and fish health. Moreover, the findings suggest that PSC can be a promising alternative protein source for sustainable aquaculture practices.2024-01-07T00:00:00Z