Analytical studies on some food contact materials and some mycotoxins in Egyptian food

Abstract

Aflatoxin M1 is the foremost metabolite of aflatoxin B1 in humans and animals, which may be present in animal products from animals fed with aflatoxin B1 contaminated feed. In this study a high performance liquid chromatography method for determination of aflatoxin M1 in dairy products and eggs was described. The solid dairy products and egg samples were diluted with warmed water and the toxin was immunoextracted followed by fluorescence detection. The average recoveries of aflatoxin M1 for milk at levels of 0.01, 0.05 and 0.2 μg/L are varied between 90.9% and 98.7%. The average recoveries of aflatoxin M1 for other matrices at the three different levels 0.05, 0.1, and 0.5 μg/kg are varied between 87% and 98%. The method is linear from the limit of quantification 0.01 up to 0.2 μg/kg levels for milk and from 0.05 to 0.5 μg/kg for other matrices. This method is novelty intended for aflatoxin M1 analyses in eggs simply with minimum toxin lose, excellent recovery, and accurate results with the limit of detection 0.01 μg/kg. The surveillance data on commercial products revealed that AFM1 is not detected in all 25 egg samples collected from different districts and yogurt is the least commodity has been contaminated with levels (8% of total samples) exceeding the national ML.Phthalate esters are the best known of endocrine disruptors so a simple and rapid method is proposed for the determination of eight types of phthalates (Dimethyl phthalate (DMP), Diethyl phthalate (DEP), Benzyl butyl phthalate (BBP), Dibutyl phthalate (DBP), Diisobutyl phthalate (DIBP), Diethylhexyl phthalate (DEHP), Dioctyl phthalate (DOP) and Diisodecyl phthalate (DIDP)) in edible oils.