The antimicrobial action of Trypsin on mature Enterococcus Faecalis Biofilm and its effect on Dentin Hardness (in vitro study)

Abstract

The aim of this study was to evaluate the antibacterial action of Trypsin on mature enterococcus faecalis biofilm model and its effect on dentin hardness. A total of fifty seven human anterior single rooted teeth (resulting in seventy two samples) were used in this study. Teeth were decoronated to length 15 mm, cleaned and shaped. Samples were divided according to the test; for antibacterial testing forty two teeth were used, for biofilm dissolution test ten teeth sectioned into halves (n=20) were used with extra four halves for investigating biofilm development and for dentin hardness test five teeth sectioned into halves (n=10) were used. Forty whole roots and 20 halves were incubated with previously cultured Enterococcus faecalis to form a biofilm for six weeks in a 37 ºc incubator. Four samples for evaluating biofilm development were scanned using SEM at two time intervals two and six weeks. At the end of the incubation time all teeth were taken out of incubated media and subjected to the planned medication either trypsin 0.5 and 5 % for biofilm dissolution evaluation or trypsin 5 % and TAP for antibacterial testing.