ENVIRONMENTAL AND BIOLOGICAL ASSESSMENT OF CYPERMETHRIN PESTICIDE ON EXPOSED WORKERS

Abstract

Cypermethrin (CYP) is a synthetic pyrethroid II insecticide, used worldwide in different agricultural uses. Chronic human exposure to CYP was associated with many adverse events. This study was conducted to evaluate CYP human exposure adverse events biochemically, hematological and immunologically. Leukocytosis and thrombocytosis were common hematological (non- significantly changed) (P>0.05). Biochemical parameters considered namely SOD, CAT, GSH, GSSG, GPx and TNO levels were determined revealing significant decrease (P<0.05-0.001) compared to normal control samples. Immunophenotyping of T cell subtypes was performed using flow-cytometery. CD3 was significantly elevated in indirectly exposed group (IDEG) (P< 0.05) compared to normal group, while CD4 showed non-significant changes in both (PEG) and (IDEG) compared to normal control , main while DEG showed a significant decrease in CD4 value compared to normal group (P<0.05). Also, CD8 count was significantly elevated in both PEG and IDEG respectively compared to normal group ,(P<0.05). Also, CD4/CD8 ratio was significantly reduced in case of IDEG and PEG respectively compared to normal group, (P<0.05- P<0.001). P53 gene sequencing and mutational changes were traced, whereas only P53 gene exon 5 showed pattern of alteration with point replacement mutation at 114 T A ; (3/184 = 0.016%) in case of DEG and (2/184 = 0.015 %) in case of PEG, while remaining P53 gene exons 6, 7 and 8 showed no pattern of changes for remaining exposed groups.