ASSESSMENT OF CURATIVE EFFECT OF USING STEM CELLS ON THE CONTROL OF PESTICIDE DAMAGING EFFECT ON LIVER BY USING IMMUNOHISTOCHEMICAL METHODS

Abstract

Introduction: Liver failure is a growing health problem and one of the main causes of death worldwide. Regenerative medicine as stem cells therapy is a newly rapidly developing field where the diseased tissues regenerated. Aim: To evaluate the effectiveness of bone marrow derived mesenchymal stem cell on liver degeneration and fibrosis induced by Chlorpyrifos and comparing its efficacy with silymarin and colchicine as antifibrotic agents used in liver fibrosis. Methods: Induction of liver degeneration and fibrosis by Chlorpyrifos (CPF) for 8 weeks to Swiss albino mice in randomly divided groups (B, C and D) and a control healthy group (A), each group composed of 15 mice. Group (B) Chlorpyrifos group (diseased not treated), Group (C) was treated with oral silymarin and colchicine and group (D) was treated with intra-peritoneal injection of Mesenchymal stem cells (MSC) (106 cells / mouse) after induction of liver fibrosis by CPF. After 4 weeks of treatment, all mice were scarified, blood was collected for chemical analysis (liver functions tests: Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Albumin, Bilirubin). Fibrosis index in liver tissue were assessed histopathologically by: H& E stain for evaluation and grading, computed quantitative morphometric measurements were performed for Sirius red stained slides to determine the percentage of collagen (type I, III), and immunohistochemical staining "[Matrix metalloproteinase-2 )MMP -2) and its tissue inhibitor (TIMP -2)]" for detection of fibrosis. Results: ALT, AST and Albumin were significant