STUDIES ON CITRUS STUBBORN DISEASE
Ayman Faisal Abdo Omar;
Abstract
Stubborn disease caused by Spiroplasma citri Saglio et al. is one of the most serious diseases of citrus in Egypt.
A survey of the disease and biological, serological and chemical studies on the pathogen was carried out in this work.
A survey was carried out to study the spread of the stubborn disease on citrus in different governorates. The highest percentage of infection was recorded in different governorates such as El-Qualubia (32.2%), followed by El-Gharbia (20.76) and Kafr El-Sheikh (16.43%).
Spiroplasma citri was isolated from common orange (Citrus sinensis Osb.) trees showing typical symptoms of stubborn disease in Kafr El-Sheikh, Egypt. The organism was isolated from leaves, stalks and seeds. Change in colour of the inoculated medium from red to yellow indicated the presence of S. citri in the tested samples. Typical fried-egg shape colonies were obtained when S. citri was subculture on solid medium. When four different sources of serum (Bovine, Calf, Horse and Donkey serum) were used, there was no obvious effect on S. citri growth.
Antisera were prepared in this work against S citri by intravenous and intramuscular injections.
The efficiency of intravenous and intramuscular injections m raising the titer of anti S. citri serum was studied using the agglutination test and ELISA. Results show that intramuscular injection produced antiserum with a titer obviously higher than antiserum produced by intravenous injection. Also, the obtained data indicate that ELISA test was more sensitive than agglutination test.
A survey of the disease and biological, serological and chemical studies on the pathogen was carried out in this work.
A survey was carried out to study the spread of the stubborn disease on citrus in different governorates. The highest percentage of infection was recorded in different governorates such as El-Qualubia (32.2%), followed by El-Gharbia (20.76) and Kafr El-Sheikh (16.43%).
Spiroplasma citri was isolated from common orange (Citrus sinensis Osb.) trees showing typical symptoms of stubborn disease in Kafr El-Sheikh, Egypt. The organism was isolated from leaves, stalks and seeds. Change in colour of the inoculated medium from red to yellow indicated the presence of S. citri in the tested samples. Typical fried-egg shape colonies were obtained when S. citri was subculture on solid medium. When four different sources of serum (Bovine, Calf, Horse and Donkey serum) were used, there was no obvious effect on S. citri growth.
Antisera were prepared in this work against S citri by intravenous and intramuscular injections.
The efficiency of intravenous and intramuscular injections m raising the titer of anti S. citri serum was studied using the agglutination test and ELISA. Results show that intramuscular injection produced antiserum with a titer obviously higher than antiserum produced by intravenous injection. Also, the obtained data indicate that ELISA test was more sensitive than agglutination test.
Other data
| Title | STUDIES ON CITRUS STUBBORN DISEASE | Other Titles | دراسات على المرض العنيد فى الموالح | Authors | Ayman Faisal Abdo Omar | Issue Date | 1999 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| B11574.pdf | 996.23 kB | Adobe PDF | View/Open |
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