Lactoferrin Assessment In Follicular Fluid And Embryo Quality And Pregnancy Rate During In Vitro Fertilization Cycles
Nahla Nazieh EL Sheikh;
Abstract
Lactoferrin is an iron-binding glycoprotein originally isolated from milk. It is present in detectable amounts in FF and it was found to be significantly more concentrated in larger follicles yielding good quality oocytes able to allow fertilization and development to good quality embryos.
In this study, we analyzed the lactoferrin concentrations in follicular fluid obtained at the time of oocyte retrieval, and we examined whether high lactoferrin concentrations in FF correlate with a good embryo quality and a higher clinical pregnancy rate.
It was an observational study including 80 participants who are assigned for treatment of infertility by ICSI technique and it was carried out at assisted reproduction unit, Ain Shams University maternity hospital.
Follicular fluid samples were collected during aspiration of the mature follicles and matched to their corresponding oocytes and it was stored immediately at -20ºC until subsequent measurement of lactoferrin concentrations using a highly sensitive two site enzyme linked immunoassay (ELISA).
Immediately after retrieval, nuclear maturation of oocytes was determined by identification of the presence of the first polar body and the detection of the germinal vesicle breakdown.
Morphology of the dividing embryos was observed and quality of the embryos from each participant was assessed immediately before embryo transfer and was correlated with follicular fluid lactoferrin concentrations.
Biochemical pregnancy: Serum ß-hCG was determined for all participants 14 days after embryo transfer, and 2 weeks later. Clinical pregnancy was confirmed by the transvaginal ultrasonographic appearance of an intrauterine gestational sac and fetal pulsations.
The current study showed that there was no statistically significant difference between the group of woman who achieved and the group of women who didn't achieve biochemical and clinical pregnancy as regards age, BMI and duration of infertility.
In addition, there was no statistically significant difference between both groups as regards number of follicles ≥18 mm, number of retrieved oocytes and number of mature MII oocytes.
The present study showed that there is statistically highly significant difference between both groups regarding number of fertilized oocytes and number of transferred embryos.
The present study showed that there was no statistically significant difference between both groups as regards embryo grades.
In this study, we analyzed the lactoferrin concentrations in follicular fluid obtained at the time of oocyte retrieval, and we examined whether high lactoferrin concentrations in FF correlate with a good embryo quality and a higher clinical pregnancy rate.
It was an observational study including 80 participants who are assigned for treatment of infertility by ICSI technique and it was carried out at assisted reproduction unit, Ain Shams University maternity hospital.
Follicular fluid samples were collected during aspiration of the mature follicles and matched to their corresponding oocytes and it was stored immediately at -20ºC until subsequent measurement of lactoferrin concentrations using a highly sensitive two site enzyme linked immunoassay (ELISA).
Immediately after retrieval, nuclear maturation of oocytes was determined by identification of the presence of the first polar body and the detection of the germinal vesicle breakdown.
Morphology of the dividing embryos was observed and quality of the embryos from each participant was assessed immediately before embryo transfer and was correlated with follicular fluid lactoferrin concentrations.
Biochemical pregnancy: Serum ß-hCG was determined for all participants 14 days after embryo transfer, and 2 weeks later. Clinical pregnancy was confirmed by the transvaginal ultrasonographic appearance of an intrauterine gestational sac and fetal pulsations.
The current study showed that there was no statistically significant difference between the group of woman who achieved and the group of women who didn't achieve biochemical and clinical pregnancy as regards age, BMI and duration of infertility.
In addition, there was no statistically significant difference between both groups as regards number of follicles ≥18 mm, number of retrieved oocytes and number of mature MII oocytes.
The present study showed that there is statistically highly significant difference between both groups regarding number of fertilized oocytes and number of transferred embryos.
The present study showed that there was no statistically significant difference between both groups as regards embryo grades.
Other data
| Title | Lactoferrin Assessment In Follicular Fluid And Embryo Quality And Pregnancy Rate During In Vitro Fertilization Cycles | Other Titles | تقييم اللكتوفرين فى السائل الحويصلى و علاقته بتقييم جودة الجنين و معدل الحمل خلال دورات الإخصاب خارج الرحم | Authors | Nahla Nazieh EL Sheikh | Issue Date | 2015 |
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