Potential anti-fibrotic effect of Pinocembrin on experimentally-induced liver fibrosis in rats
Marwa Mohammad Said Abdelazim;
Abstract
Liver fibrosis is a disease that has no effective treatment so far.
Therefore, there are many models that cause hepatic toxicity to be
used to test substances that may have an effective influence in the
treatment of fibrosis. Among these models, CCl4 model which is widely
used in this area. PIN is a major flavanone found in honey and propolis
and possesses antioxidant, anti-inflammatory and pulmonary antifibrotic
effect. The present study aims to investigate the potential
protective effect of PIN against CCl4-induced liver fibrosis in rats and
to clarify the underlying mechanism by studying its effect on different
oxidative stress, inflammatory, as well as fibrotic markers.
This study was divided into two parts:
1. Screening the potential hepatoprotective dose of PIN:
The dose response study was constructed using different
dosages of PIN. Rats were divided into 5 groups:
Group (I) (considered as the control group): was given 1ml/kg of
HPβCD through oral gavage once daily for 7 consecutive days.
Group (II) )CCl4 group(: was given 1ml/kg of HPβCD through oral
gavage once daily for 7 consecutive days followed by a single i.p.
injection of CCl4 (1 ml/kg, 1:1 mixture with corn oil) on the 5
th
day.
Groups (III, IV and V) were PIN pretreated groups, were given 10, 20
and 40 mg/kg of PIN respectively through oral gavage once daily for
7 consecutive days followed by a single i.p. injection of CCl4 (1
ml/kg, 1:1 mixture with corn oil) on the 5
th
day 1 h after PIN treatment.
On day 8, blood samples were collected then animals of all
groups were sacrificed, and liver tissues were dissected out for
histopathological examination.
123
Therefore, there are many models that cause hepatic toxicity to be
used to test substances that may have an effective influence in the
treatment of fibrosis. Among these models, CCl4 model which is widely
used in this area. PIN is a major flavanone found in honey and propolis
and possesses antioxidant, anti-inflammatory and pulmonary antifibrotic
effect. The present study aims to investigate the potential
protective effect of PIN against CCl4-induced liver fibrosis in rats and
to clarify the underlying mechanism by studying its effect on different
oxidative stress, inflammatory, as well as fibrotic markers.
This study was divided into two parts:
1. Screening the potential hepatoprotective dose of PIN:
The dose response study was constructed using different
dosages of PIN. Rats were divided into 5 groups:
Group (I) (considered as the control group): was given 1ml/kg of
HPβCD through oral gavage once daily for 7 consecutive days.
Group (II) )CCl4 group(: was given 1ml/kg of HPβCD through oral
gavage once daily for 7 consecutive days followed by a single i.p.
injection of CCl4 (1 ml/kg, 1:1 mixture with corn oil) on the 5
th
day.
Groups (III, IV and V) were PIN pretreated groups, were given 10, 20
and 40 mg/kg of PIN respectively through oral gavage once daily for
7 consecutive days followed by a single i.p. injection of CCl4 (1
ml/kg, 1:1 mixture with corn oil) on the 5
th
day 1 h after PIN treatment.
On day 8, blood samples were collected then animals of all
groups were sacrificed, and liver tissues were dissected out for
histopathological examination.
123
Other data
| Title | Potential anti-fibrotic effect of Pinocembrin on experimentally-induced liver fibrosis in rats | Other Titles | التأثيرالمضاد للتليف المحتمل لمركب بينوسيمبرين على تليف الكبد المحدث تجريبياً في الجرذان | Authors | Marwa Mohammad Said Abdelazim | Issue Date | 2017 |
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