Evaluation of Chromogenic medium Chrom ID OXA-48 for detection of OXA-48 producing Enterobacteriaceae in Cairo

Abstract

Carbapenemase producing Gram-negative bacteria have been recognised as an increasing concern over the past decade, following global spread. The early detection of carbapenemase producing organisms will allow early effective antibiotic therapy, improve antibiotic stewardship and allow infection control procedures to be initiated. Many studies have established that OXA-48 is the most frequently encountered carbapenemase in Enterobacteriaceae. K pneumoniae is reported to be the dominant causative organism of outbreaks of OXA-48 CPE. Chromogenic media may facilitate identification of microorganisms and may promote easy interpretation of culture plates according to chromogenic substrates which enable the identification of most frequently isolated CPE. The present study evaluated a chromogenic agar ChromID OXA-48 (bioMérieux) for detection of OXA-48 carbapenemase producing Enterobacteriaceae compared to detection of bla OXA-48 gene by PCR.