A Clinical and Biochemical Assessment of Interleukin-17 Level in Gingival Crevicular Fluid in Patients with Periodontal Disease before and after Periodontal Treatment
Tarek Mahmoud EL-Tayeb;
Abstract
riodontal disease is a bacteria-induced chronic inflammatory disease. It affects the soft and hard supporting structures encompassing the teeth.
Several proinflammatory cytokines and chemokines, responsible for tissue destruction are secreted in GCF, it possess a great potential for serving as diagnostic or prognostic markers of the periodontal health, disease and healing after therapy. The collection of GCF is a relatively simple, noninvasive, and site specific procedure.
Interleukin-17 (IL-17A) was originally described and cloned by Rouvier et al. (1993) and named CTLA8 and was subsequently renamed IL-17, and more recently, IL-17A. The gene location for IL-17A is 6p12, and IL-17A shows 58% homology with an open reading frame of the T lymphotropic herpesvirus samirii dimer (viral IL-17). IL-17A is the prototypic IL-17 family member in that it is a disulfide-linked homodimeric glycoprotein consisting of 155 amino acids, exerting its actions as a homodimer with a molecular weight around 35 kDa.
IL-17 is a pro-inflammatory cytokine derived from T cells and produced mainly by cells of the Th1/Th0 phenotype but not cells of the Th2 phenotype. Recent findings have defined IL-17-producing cells as a new Th cell lineage, renamed Th17. The discovery of Th17 cells has been one of the most important advances in T cell immunology since the discovery of Th1 and Th2 cells by Mosmann, Coffman, and their colleagues more than two decades ago.
Homology-based cloning has recently revealed five additional
IL-17 family members, termed IL-17B to IL-17F. These molecules also form homodimers and show conservation in their c-terminal region, with five spatially conserved cysteine residues accounting for a characteristic cysteine-knot formation for IL-17A and IL-17F.
Interleukin-17 in the GCF was positively correlated to the severity of periodontitis stratified by probing pocket depth, which is the most widely used diagnostic tool for the clinical assessment of connective tissue and bone destruction in periodontitis. Also, there was decreased level of interleukin-17 after non-surgical periodontal therapy.
The study was conducted on forty subjects. They were divided into three groups based on probing pocket depth (PPD) and clinical attachment level (CAL): chronic periodontitis (group 1) (n=15), generalized aggressive periodontitis (group 2) (n=15) and healthy control (group 3) (n=10). Clinical examination including plaque index, gingival index, probing pocket depth and clinical attachment level was carried at the baseline and three months after non surgical periodontal therapy.
GCF samples were collected from all the groups to estimate the levels of interleukin-17 using ELISA at baseline and three months after non surgical periodontal therapy i.e. scaling and root planing (SRP).
The results of interleukin-17 in GCF were compared in the three groups before and after treatment. The mean interleukin-17 at baseline was highest in aggressive periodontitis group (57.47±18.66). The mean
Several proinflammatory cytokines and chemokines, responsible for tissue destruction are secreted in GCF, it possess a great potential for serving as diagnostic or prognostic markers of the periodontal health, disease and healing after therapy. The collection of GCF is a relatively simple, noninvasive, and site specific procedure.
Interleukin-17 (IL-17A) was originally described and cloned by Rouvier et al. (1993) and named CTLA8 and was subsequently renamed IL-17, and more recently, IL-17A. The gene location for IL-17A is 6p12, and IL-17A shows 58% homology with an open reading frame of the T lymphotropic herpesvirus samirii dimer (viral IL-17). IL-17A is the prototypic IL-17 family member in that it is a disulfide-linked homodimeric glycoprotein consisting of 155 amino acids, exerting its actions as a homodimer with a molecular weight around 35 kDa.
IL-17 is a pro-inflammatory cytokine derived from T cells and produced mainly by cells of the Th1/Th0 phenotype but not cells of the Th2 phenotype. Recent findings have defined IL-17-producing cells as a new Th cell lineage, renamed Th17. The discovery of Th17 cells has been one of the most important advances in T cell immunology since the discovery of Th1 and Th2 cells by Mosmann, Coffman, and their colleagues more than two decades ago.
Homology-based cloning has recently revealed five additional
IL-17 family members, termed IL-17B to IL-17F. These molecules also form homodimers and show conservation in their c-terminal region, with five spatially conserved cysteine residues accounting for a characteristic cysteine-knot formation for IL-17A and IL-17F.
Interleukin-17 in the GCF was positively correlated to the severity of periodontitis stratified by probing pocket depth, which is the most widely used diagnostic tool for the clinical assessment of connective tissue and bone destruction in periodontitis. Also, there was decreased level of interleukin-17 after non-surgical periodontal therapy.
The study was conducted on forty subjects. They were divided into three groups based on probing pocket depth (PPD) and clinical attachment level (CAL): chronic periodontitis (group 1) (n=15), generalized aggressive periodontitis (group 2) (n=15) and healthy control (group 3) (n=10). Clinical examination including plaque index, gingival index, probing pocket depth and clinical attachment level was carried at the baseline and three months after non surgical periodontal therapy.
GCF samples were collected from all the groups to estimate the levels of interleukin-17 using ELISA at baseline and three months after non surgical periodontal therapy i.e. scaling and root planing (SRP).
The results of interleukin-17 in GCF were compared in the three groups before and after treatment. The mean interleukin-17 at baseline was highest in aggressive periodontitis group (57.47±18.66). The mean
Other data
| Title | A Clinical and Biochemical Assessment of Interleukin-17 Level in Gingival Crevicular Fluid in Patients with Periodontal Disease before and after Periodontal Treatment | Other Titles | تقييم مستوى انترلوكين 17 في السائل اللثوي في التهاب الأنسجة الداعمة الحول سنية قبل وبعد علاج اللثه | Authors | Tarek Mahmoud EL-Tayeb | Issue Date | 2014 |
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