The Effect of Finishing Techniques and Time on Surface Roughness and Biocompatibility of Resin Modified Glass Ionomer Cements
Heba Mohammed Mohammed Abdelaal;
Abstract
Section (1):
An in-vitro study was performed to evaluate the effect of finishing techniques and time of finishing on the surface roughness of three types of resin modified glass ionomer cements.
A total of 150 resin modified glass ionomer cement specimens prepared from specially constructed split Teflon molds of five mm diameter and three mm thickness were used for this study. The specimens were divided into three main groups (n=50) according to the type of resin-modified glass ionomer cement used ( Fuji VIII, Fuji II LC and Ketac Nano). Each main group was divided into five groups (n=10) according to the type of finishing technique used (Mylar strip as control, Jiffy rubber tips, Sof-lex aluminum oxide discs, diamond abrasive and diamond abrasive followed by Equia coat). Each group was further divided into two subgroups (n=5) according to the time of finishing (after 24 hours or after one week). After application of the different finishing protocols, Environmental Scanning Electron Microscope (ESEM, quanta200,FEI) was used for evaluation of the surface roughness of the specimens.
The surface roughness results showed the resin modified glass ionomer material and the finishing technique had a statistically significant effect on the surface roughness (p=.000), while the time of finishing had no significant effect on the surface roughness (p=.775).
Section (2):
An in-vivo study was performed to evaluate the effect of finishing techniques and time of finishing on the biocompatibility of the same types of resin modified glass ionomer cements.
A total of 300 specimens (5mm × 3mm) were prepared for bio-compatibility evaluation. The specimens were divided into two main groups (n=150) according to the period of histological evaluation of inflammation (after one month and after two months). Each main group was divided into three groups (n=50) according to the type of resin-modified glass ionomer cement used (Fuji VIII, Fuji II LC and Ketac nano). Each group was divided into five groups (n=10) according to the type of finishing technique used ( Mylar strip as control, Jiffy rubber tips, Sof-lex aluminum oxide discs, diamond abrasive and diamond abrasive followed by Equia coat). Each group was further divided into two subgroups (n=5) according to the time of finishing (after 24 hours or after one week).
After application of the different finishing protocols, the RMGIC specimens were implanted into the subcutaneous connective tissue of the dorsal surfaces of 60 male albino rats. The same surgical procedures were done for 10 albino rats but without the implantation of the tested specimens to serve as negative control. The histological evaluations were performed at, 30 days and 60 days post implantation. Histomorphometric Analysis was done using the Image J software to calculate the number of inflammatory cells.
Histopathologic observation of the implanted sites at each time-period showed that RMGIC implants induced foreign body reaction, showing intense to moderate inflammatory reactions in most cases, which diminished significantly with time with the formation of a capsule. While a minimalinflammatory response was observed in unimplanted control tissues.
The statistical analysis of the results showed that the finishing technique and the time of finishing had a statistically significant effect on the mean number of inflammatory cells in both evaluation periods (p=.000), while the resin modified glass ionomer material had no significant effect on the surface roughness.
Under the circumstances of this study, the following conclusions could be suggested:
1. The silicon-impregnated rubber and Aluminum oxide disc finishing modalities directly improve the surface finish, technique wise, whereas Abrasive point finishing modality dramatically affects the surface profile.
2. The mode of curing (chemical, dual and tricure) has a great and direct relation on the mean surface roughness profile with emphasis to the time lapse.
3. Changes of the surface roughness character of the samples, in relation to finishing technique and time of finishing, have a direct relation on subcutaneous tissue reaction, with interest inflammatory cell count.
4. Improved tissue tolerance to the tested RMGICs appears to be time-dependent.
An in-vitro study was performed to evaluate the effect of finishing techniques and time of finishing on the surface roughness of three types of resin modified glass ionomer cements.
A total of 150 resin modified glass ionomer cement specimens prepared from specially constructed split Teflon molds of five mm diameter and three mm thickness were used for this study. The specimens were divided into three main groups (n=50) according to the type of resin-modified glass ionomer cement used ( Fuji VIII, Fuji II LC and Ketac Nano). Each main group was divided into five groups (n=10) according to the type of finishing technique used (Mylar strip as control, Jiffy rubber tips, Sof-lex aluminum oxide discs, diamond abrasive and diamond abrasive followed by Equia coat). Each group was further divided into two subgroups (n=5) according to the time of finishing (after 24 hours or after one week). After application of the different finishing protocols, Environmental Scanning Electron Microscope (ESEM, quanta200,FEI) was used for evaluation of the surface roughness of the specimens.
The surface roughness results showed the resin modified glass ionomer material and the finishing technique had a statistically significant effect on the surface roughness (p=.000), while the time of finishing had no significant effect on the surface roughness (p=.775).
Section (2):
An in-vivo study was performed to evaluate the effect of finishing techniques and time of finishing on the biocompatibility of the same types of resin modified glass ionomer cements.
A total of 300 specimens (5mm × 3mm) were prepared for bio-compatibility evaluation. The specimens were divided into two main groups (n=150) according to the period of histological evaluation of inflammation (after one month and after two months). Each main group was divided into three groups (n=50) according to the type of resin-modified glass ionomer cement used (Fuji VIII, Fuji II LC and Ketac nano). Each group was divided into five groups (n=10) according to the type of finishing technique used ( Mylar strip as control, Jiffy rubber tips, Sof-lex aluminum oxide discs, diamond abrasive and diamond abrasive followed by Equia coat). Each group was further divided into two subgroups (n=5) according to the time of finishing (after 24 hours or after one week).
After application of the different finishing protocols, the RMGIC specimens were implanted into the subcutaneous connective tissue of the dorsal surfaces of 60 male albino rats. The same surgical procedures were done for 10 albino rats but without the implantation of the tested specimens to serve as negative control. The histological evaluations were performed at, 30 days and 60 days post implantation. Histomorphometric Analysis was done using the Image J software to calculate the number of inflammatory cells.
Histopathologic observation of the implanted sites at each time-period showed that RMGIC implants induced foreign body reaction, showing intense to moderate inflammatory reactions in most cases, which diminished significantly with time with the formation of a capsule. While a minimalinflammatory response was observed in unimplanted control tissues.
The statistical analysis of the results showed that the finishing technique and the time of finishing had a statistically significant effect on the mean number of inflammatory cells in both evaluation periods (p=.000), while the resin modified glass ionomer material had no significant effect on the surface roughness.
Under the circumstances of this study, the following conclusions could be suggested:
1. The silicon-impregnated rubber and Aluminum oxide disc finishing modalities directly improve the surface finish, technique wise, whereas Abrasive point finishing modality dramatically affects the surface profile.
2. The mode of curing (chemical, dual and tricure) has a great and direct relation on the mean surface roughness profile with emphasis to the time lapse.
3. Changes of the surface roughness character of the samples, in relation to finishing technique and time of finishing, have a direct relation on subcutaneous tissue reaction, with interest inflammatory cell count.
4. Improved tissue tolerance to the tested RMGICs appears to be time-dependent.
Other data
| Title | The Effect of Finishing Techniques and Time on Surface Roughness and Biocompatibility of Resin Modified Glass Ionomer Cements | Other Titles | تأثير طرق ووقت الصقل على خشونة السطح و التوافق البيولوجى للاصق الأيونومر الزجاجى المعدل راتنجيا | Authors | Heba Mohammed Mohammed Abdelaal | Issue Date | 2015 |
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