PRODUCTION OF VIRUS-FREE PLANTS
SAMEH DIF ALLAH THABET ATTYA;
Abstract
Banana btmchy top virus (BBTV) and banana mosaic virus (BMV) were isolated from natural infected banana plants. •
BBTV and BMV were partially purified using 10% polyethylene glycol and I% NaCI and 6% PEG and 2% NaCl respectively and then highly purified using either differential centrifugation, or gel electrophoresis. The virus yield was 0.11 mg/kg infected tissue in case of BBTV, while in case of BMV it was 3.156 mg/kg infected tissue.
Electron microscopy examination showed that BBTV &
BMV appeared to be spherical particles with 18-20 run and 27-
29 run in diameter, respectively.
The induced antisera ofliighly purified BBTV and BMV using differential centrifugation and gel electrophoresis methods showed positive reaction against BBTV and BMV when' examined using agar double diffusion test (Ouchterlony), and double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA).
PART II: Factors affecting production of virus-free plants.
Meristems of infected banana plants with BBTV and BMV were exposed to different treatments : meristem culture, thermotherapy and chemotherapy in order to obtained virus-free bm1ana plantlets.
BBTV and BMV were partially purified using 10% polyethylene glycol and I% NaCI and 6% PEG and 2% NaCl respectively and then highly purified using either differential centrifugation, or gel electrophoresis. The virus yield was 0.11 mg/kg infected tissue in case of BBTV, while in case of BMV it was 3.156 mg/kg infected tissue.
Electron microscopy examination showed that BBTV &
BMV appeared to be spherical particles with 18-20 run and 27-
29 run in diameter, respectively.
The induced antisera ofliighly purified BBTV and BMV using differential centrifugation and gel electrophoresis methods showed positive reaction against BBTV and BMV when' examined using agar double diffusion test (Ouchterlony), and double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA).
PART II: Factors affecting production of virus-free plants.
Meristems of infected banana plants with BBTV and BMV were exposed to different treatments : meristem culture, thermotherapy and chemotherapy in order to obtained virus-free bm1ana plantlets.
Other data
| Title | PRODUCTION OF VIRUS-FREE PLANTS | Other Titles | إنتاج نباتات خالية من الفيروس | Authors | SAMEH DIF ALLAH THABET ATTYA | Issue Date | 2000 |
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