ASSOCIATION BETWEEN PORPHYROMONAS GINGIVALIS AND PREECLAMPSIA

Abstract

The association between periodontitis and some of the problems with pregnancy such as premature delivery, low weight at birth, and preeclampsia (PE) has been suggested . This case-control study was done at Ain Shams University Maternity Hospital, between Aprill 2015 and Septemper 2015; to study the association between Porphyromonas gingivalis and Preeclampsia .A total of 48 patients, having single intra-uterine fetus, were included in the study . Subjects were selected from those attending the delivery unit and were scheduled for elective cesarean delivery.They were divided into 2 groups;24 patients in each . Group (1) were suffering from preeclampsia and group (2) were normotensive . Pregnant women with any associated medical disorder and patients who used systemic antimicrobial therapy were excluded from the study . History and oral examination: A full history was taken from each patient and oral examination was performed for each patient by a single periodontist prior to cesarean delivery. Participants were grouped according to periodontal status into ; normal healthy gingiva ,gingivitis and periodontitis . Specimens : Sub-gingival plaque and placental samples were collected from each patient and transported to polymerase chain reaction (PCR) section within 4 hours of collection . The samples were stored at -60°C until PCR analysis . Total DNA from placental and subgingival samples was extracted by using the QIAamp Tissue Kits (Qiagen, Hilden, Germany ) according to the manufacturer's instructions . Ethical approval : The study was approved by ethical and research committee of council of Obstetrics and Gynaecology Department ,Ain Shams University at April 2015 . Results: There were no significant statistical differences between the two groups as regards maternal characteristics. But, there were significant statistical differences between the two groups as regards neonatal birth weight which was lower in preeclmpsia group . There were significant statistical differences between the two groups as the proportion of women who had positive P.gingivalis in placental samples were higher in preeclampsia group (group 1) when compared to the normotensive control group (group 2) with statistical significance .