Antibacterial activity and protein analysis (proteomic approach) of Oriental Hornet Vespa orientalis L. Venom

Abstract

Antibacterial activity of venom extracted from the oriental hornet Vespa orientalis L. was tested against different bacterial pathogens. The selected bacterial strains were MRSA, B.subtilis and S.muans as Gram-positive bacteria; E.coli, K.pneumonia and S. typhimurium as Gram-negative bacteria.

To identify the proteins responsible for the antibacterial activity of wasp venom an electrophoretic study was carried out.

Mode of action of wasp venom against the tested bacterial strains was studied by transmission electron microscopy.

The results of the study can be summarized as follows:

1. Wasp venom activity against Gram+ve bacteria:

In measuring the antimicrobial properties of Vespa orientalis L. venom (conc.0.2mg/ml) activity against Gram positive bacteria, it was found that Vespa orientalis L. crude venom represented a significant effect against MRSA،B.subtilis and S.mutans. The measured inhibition zones were 19.2 mm for MRSA, 18.3 mm for B.subtilis and 10.2mm for S.mutans. Comparing with the tetracycline antibiotic the measured inhibition zones were 9.9mm for MRSA, 13.6 mm for B. subtilis and 16.5mm for S.mutans.

2. Wasp venom activity against Gram -ve bacteria:

In measuring the antimicrobial activity of Vespa orientalis L. venom (conc.0.2mg/ml) activity against Gram negative bacteria, it was found that Vespa orientalis L. crude

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