The Effects of Wormwood (Artemisia monosperma A. ) Oil Extract on The Risks of Colon Cancer Induced in Rats

Abstract

Cancer is one of the main diseases in human populations where reactive oxygen species (ROS) play an important role in all major stages of carcinogenesis. In this point of view anti-oxidant therapy has become important for both prevention and palliative treatment of malignant diseases. Hence, this study was conducted to investigate the anti-oxidant capacity, the biological and biochemical impact of the oil extract of Artemisia monosperma A. (wormwood, WO) extract to alleviate and improve the colon cancer induced chemically in male adult albino rats. This study was divided into two sections:- The first section consists of analysis of the phytochemicals constituents and the percentage of polyunsaturated fatty acids as well as anti-oxidant activity of WO extract. Data presented that each 100 g of the prepared oil extract contained 237 ± 2.3 mg as gallic acid equivalent for total phenols, 3.06 ± 0.01 mg as catechin equivalent for flavonoids, 11.34 ± 0.68 mg of carotenoids, 1.95 ± 0.06 mg as alkaloids and polyunsaturated fatty acid represent 66.16 % of fatty acids. The tested oil extract also showed high free radical scavenging activity of 1, 1-diphenyl-2-picryl hydrazyl (DPPH) radicals by about 95.6 %. The second section consisted of the biological trial. Throughout this study, sixty of healthy male adult albino rats Spargue-Dawley strain weighing 190 ± 10 g were subjected to experimentation conditions. All rats were offered the balanced diet with drinking water ad libitum. Animals were divided into 6 groups 10 rats each: (group 1): Healthy rats (control group), (group 2): Healthy rats consumed WO extract daily at dose (600