Evaluation of Quantitative Immunomagnetic Bead ELISA technique Using Super-Paramagnetic Nanoparticles in Diagnosis of Schistosomiasis haematobium

Abstract

Immunodiagnosis of urinary schistosomiasis has proved to be very useful and more sensitive than parasitological methods. Antigen detection assays are considered of prime importance for immunodiagnosis, as the detection of circulating antigens can indicate an active infection. This study included 180 humans divided in 3 groups, Group A: Schistosoma infected group (n=60), group B: other parasites infected group (n=60) and group C: healthy control group (n=60). Schistosomiasis infected patients were subjected to clinical history taking, parasitological estimation of infection intensity, and detection of proteinuria and haematuria. The urine and serum samples of all the study groups were examined for the presence of CL antigen by sandwich ELISA procedure using rabbit polyclonal IgG anti-Schistosoma antibodies. Concerning the main presenting symptoms of the Schistosoma infected patients 65% gave a complaint while 35% of patients were asyptomatic. Macroscopic haematuria was present in 25% of patients, dysuria was present in 20% and 20% show both haematuria and dysuria.