IMMUNOHISTOCHEMICAL EVALUATION OF ANGIOGENESIS IN PRIMARY BLADDER CARCINOMA
Abeer Moneer Mostafa;
Abstract
This study was performed on 30 patients with primary bladder carcinomas admitted at Ain Shams Specialized hospital in the period between February
1998 to January 2000. The aim of the study was to assess the tumor angiogenesis in different types of primary bladder carcinomas through immunohistochemical expression of CD34 antibody in these tumors and its correlation with their histologic type, tumor grading, and clinical staging.
Thirty paraffm embedded specimens were chosen 19 transitional cell carcmoma, 9 squamouas cell carcinoma, 2 sarcomatoid. Their ages range between
60 and more than 90 years. Twenty eight were males and two were females. All data regarding age, sex, history of Bilharziasis and tumor stage were obtained from patient's files.
All specimens were cut at 3 urn, Two slides were obtained from the same block containing the highest tumor density. One slide stained with hematoxylin and eosin to confirm the histologic type and the nuclear grading. The other slide was immunohistochemically stained with CD34 antibody for quantitative analysis of angiogenic blood vessel density. CD34 stained specllllens were examined under light microscope. Five microscopic fields were counted to calculate the mean vessel count.
1998 to January 2000. The aim of the study was to assess the tumor angiogenesis in different types of primary bladder carcinomas through immunohistochemical expression of CD34 antibody in these tumors and its correlation with their histologic type, tumor grading, and clinical staging.
Thirty paraffm embedded specimens were chosen 19 transitional cell carcmoma, 9 squamouas cell carcinoma, 2 sarcomatoid. Their ages range between
60 and more than 90 years. Twenty eight were males and two were females. All data regarding age, sex, history of Bilharziasis and tumor stage were obtained from patient's files.
All specimens were cut at 3 urn, Two slides were obtained from the same block containing the highest tumor density. One slide stained with hematoxylin and eosin to confirm the histologic type and the nuclear grading. The other slide was immunohistochemically stained with CD34 antibody for quantitative analysis of angiogenic blood vessel density. CD34 stained specllllens were examined under light microscope. Five microscopic fields were counted to calculate the mean vessel count.
Other data
| Title | IMMUNOHISTOCHEMICAL EVALUATION OF ANGIOGENESIS IN PRIMARY BLADDER CARCINOMA | Other Titles | تقييم تكوين الأوعية الدموية بصبغة المناعة الكيميائية للأنسجة فى الأورام الخبيثة الأولية للمثانة | Authors | Abeer Moneer Mostafa | Issue Date | 2000 |
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