STUDY OF APOPTOSIS AND Ki-67 PROLIFERATIVE ANTIGEN IN CHRONIC GASTRITIS, HELICOBACTER AND NON- HELICOBACTER TYPES

Abstract

Since the isolation of Helicobacter pylori by Warren and Marshall in 1983, much evidence has accumulated to show its important ro)in the pathogenesis of chronic gastritis, peptic ulcer and its possible role in gastric cancer. During the multistep process of carcinogenesis, the regulation of cell proliferation and apoptosis are disturbed mostly, with enhanced cell turnover. The present study included 43 cases of endoscopic gastric biopsies, collected randomly and diagnosed as chronic gastritis. Twenty eight (65.12%) of them were H.pylori positive and 15 (34.88%) were \i'egative for the organism, moreover 5nonnal gastric biopsies were encountered as controls. The following stains were used; H&E, Giemsa and Altlah blue PAS at pH 2.5 for the graded and ungraded variables ofthe updated Sydney System. In addition Feulgen nucleal reaction and ki-67 monoclonal . antibody for detection of apoptotic events and proliferating cells respectively. '••-• It was found that, activity, lymphoid follicles and surface ep,ithelial damage were statistically significant higher in H.pylori cases'th'an non H. pylori group. In cell turnover, while the three indices (PI%, AI% and A/P ratio) were statistically significant different in both groups of gastritides compared to those of the controls, no significant difference was detected between .each other. The three indices were signd'icantly correlated with the grade of chronic inflammation and activity in both H.py/ori and non H. pylori cases, while atrophy and intestinal metaplasia it was not so.