"preparation and characterization of Nano Composites Of Chitosan with Different metal"

Abstract

I. Gold nanoparticles (AuNPs) was prepared via simple and green method by using polysaccharides as reducing and stabilizing agents at the same time. Hiren we used carboxymethylchitosan (CMCS) as a reducing agent for gold nanoparticle as well as capping agent. CMCS prepared based on our previous method by reacting chitosan with monochloroacetic acid in alkaline medium. AuNPs were prepared by using different concentrations of carboxymethylchitosan (0.2% w/v, 0.5% w/v and 1% w/v) at 100 oC for 1 hour. CMCS was characterized by using nitrogen content, carboxyl content and FTIR spectra. AuNPs was characterized by using UV spectrophotometry and TEM images. Finally, the cytotoxicity of the prepared AuNPs were evaluated using cell viability assay from