Determination and validation of some important drugs by some molecular spectrometric analysis

Abstract

Simple, rapid and sensitive spectrophotometric and chromatographic procedures were developed for the analysis of diaveridine,sulphadimidine, azithromycin, repaglinide andgranisteron hydrochloride in pure form as well as in their pharmaceutical formulations using charge transfer (CT) complexes formed between the drugs and iodine (I2) as σ- acceptor and picric acid(PA), tetracyanoethylene (TCNE) as π-acceptors in dichloromethane. Job’s method of continuous variation was used to detect the stoichiometric ratio of the formed complexes. The formation constant and molar absorptivity were determined by Benesi-Hildebrand equation. The validation of analytical procedureslike accuracy, precision, LOD, LOQ, linearity, repeatability and Robustness of drugswere determined. The formed solid charge transfer complexes between the two drugs and iodine were prepared and their structureswere investigated through elemental analysis, IR, Raman spectroscopy andNMR spectra. Also HPLC methods were developed to determine the concentrations of these drugs in bulk and pharmaceutical formulation forms.

Key words: Diaveridine, Sulphadimidine, Azithromycin, Repaglinide and Granisteron hydrochloride,charge transfer complexes, pharmaceutical analysis, spectrophotometry, HPLC.