NOVEL TRENDS FOR RAPID DETECTION OF SOME FOOD-BORNE PATHOGENS IN MILK AND SOME DAIRY PRODUCTS

Abstract

Two hundred and fifty samples (one hundred raw milk and fifty each of Domietta cheese, Kariesh cheese and ice-cream) were collected from different localities in Cairo and Giza Governorates and examined bacteriologically for prevalence of pathogenic E. coli (E. coli 0157:H7), Staphylococcus aureus and its toxins, and Listeria monocytogenes, and also using of some novel trends for isolation and identification of these pathogens.

m- Pathogenic E. coli with regard to E.coli Ow:H1. :-

The results obtained revealed that coliform was found in 99, 44, 66 and 88% of raw milk, Domietta cheese, Kariesh cheese and ice-cream

respectively with mean values of 83.30x106 ± 15.30x106

25.56x104 ±

12.44x104, 21.60x104±8.60x104 and 65.70x105 ± 53.20xl05 in the examined samples respectively. While by rapid recovery at 44°C. Escherichia coli could be detected in 17, 14, 6 and 10% with mean values of 19.70xl06 ± 9.30x106, 55.10x104 ± 13.80xl0\28.70x102 ± 17.30xl02

and 8.20x103 ± 1.60x103 in the examined samples respectively.

Enterohaemorrahagic E. coli was presumptively detected in 34, 15,

7 and 18 of the examined samples respectively, by using of Sorbitol MacConkey agar. Only 20, 9, 5 and 16 were detected by glucouronidase activity (GUD) to harbor EHEC respectively. While (3, 6, 0 and 2) and (2, 4, 0 and 2) were confirmed serologically to be E. coli Om:H7 by Latex (Oxoid) and tube agglutination (Difco) tests respectively.