Biofilm production by Pseudomonas aeruginosa clinical isolates and its relationship with pseudomonas quinolone signal (pqsA) gene and antibiotic resistance
Rania Alam El-Deen Mohamed Alaam;
Abstract
Bacteria exist, in most environments, as complex organized communities of sessile cells embedded within a self-produced matrix. These communities are known as biofilms. Bacterial biofilms contribute to more than 80% of hospital-acquired and community-acquired infections. Pseudomonas aeurginosa (P. aeruginosa) is an important example of pathogens able to form biofilm.
The capability to form biofilm by this organism is considered as a virulence factor. Biofilm-related infections are challenging to treat and difficult to fully eradicate with normal treatment regimens. Quorum sensing (QS) is important in regulating the biofilm formation.
This study aimed to determin the ability of different P. aeruginosa clinical isolates to produce biofilm and its association with pseudomonas quinolon signal A (Pqs A) gene and antibiotic resistance.
In this study 30 isolates of P. aeurginosa were obtained from different clinical samples from patients admitted to Ain Shams University Hospitals. Clinical samples from which isolates were obtained are pus, sputum and urine.
All samples were cultured on MacConkey's agar media then the isolated organisms were identified by colonial morphology, Gram staining and biochemical reactions. Antibiotic sensitivity of the isolated strains was done using disc diffusion method. The presence of Pqs A gene in isolated strains was detected using conventional PCR technique.
The isolated bacteria were tested for their ability to form biofilm using microtitre plate assay. For the biofilm forming isolates antibiotic (amikacin and meropenem) susceptibility of sessile cells minimal biofilm eradication concentration (MBEC) were tested and compared to the minimal inhibitory concentration (MIC) of their planktonic cells.
It was found that:
1. 17 out of 30 isolates (57%) were positive biofilm producers: 7% were strong biofilm producers, 27% were moderate biofilm producers and 23% were weak biofilm producers.
2. 27 out of 30 isolates (90%) had the Pqs A gene.
3. All biofilm producing strains had Pqs A gene and its presence was statistically significant to biofilm production.
4. The isolates were most sensitive to meropenem (77%) followed by amikacin (67%), while least sensitive to piperacillin (27%) and ceftazidime (7
The capability to form biofilm by this organism is considered as a virulence factor. Biofilm-related infections are challenging to treat and difficult to fully eradicate with normal treatment regimens. Quorum sensing (QS) is important in regulating the biofilm formation.
This study aimed to determin the ability of different P. aeruginosa clinical isolates to produce biofilm and its association with pseudomonas quinolon signal A (Pqs A) gene and antibiotic resistance.
In this study 30 isolates of P. aeurginosa were obtained from different clinical samples from patients admitted to Ain Shams University Hospitals. Clinical samples from which isolates were obtained are pus, sputum and urine.
All samples were cultured on MacConkey's agar media then the isolated organisms were identified by colonial morphology, Gram staining and biochemical reactions. Antibiotic sensitivity of the isolated strains was done using disc diffusion method. The presence of Pqs A gene in isolated strains was detected using conventional PCR technique.
The isolated bacteria were tested for their ability to form biofilm using microtitre plate assay. For the biofilm forming isolates antibiotic (amikacin and meropenem) susceptibility of sessile cells minimal biofilm eradication concentration (MBEC) were tested and compared to the minimal inhibitory concentration (MIC) of their planktonic cells.
It was found that:
1. 17 out of 30 isolates (57%) were positive biofilm producers: 7% were strong biofilm producers, 27% were moderate biofilm producers and 23% were weak biofilm producers.
2. 27 out of 30 isolates (90%) had the Pqs A gene.
3. All biofilm producing strains had Pqs A gene and its presence was statistically significant to biofilm production.
4. The isolates were most sensitive to meropenem (77%) followed by amikacin (67%), while least sensitive to piperacillin (27%) and ceftazidime (7
Other data
| Title | Biofilm production by Pseudomonas aeruginosa clinical isolates and its relationship with pseudomonas quinolone signal (pqsA) gene and antibiotic resistance | Other Titles | إنتاج الفيلم الحيوي بواسطة المعزولات الإكلينيكية للزائفة الزنجارية وعلاقته بجين إشارة كينولون الزائفة أ وبمقاومة المضادات الحيوية | Authors | Rania Alam El-Deen Mohamed Alaam | Issue Date | 2017 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.