STUDY OF THE HUMORAL .AND CELLULAR IMMUNE RESPONSES TO DIFFERENT FASCIOLA GIGANTICA ANTIGENS

Abstract

A group of antigens were purified from execretory-secretory antigens (CP or FABP) of Fasciola gigantic using immunoaffinity chromatography. These antigens were studied to evaluate their protective effect against fascioliasis. The CP and FABP were used before and after infection. Parasitological parameters (worm burden and bile egg count) and immunological parameters (immunoglobulin and interleukin level) were undertaken. It was evident that CP was more potent and FABP in reducing worm burden and egg count and evaluating levels of immunoglobulin isotypes (IgM, total IgG, IgG2 and IgG4) and interleukins (IL-6, IL-10, IL-12 and TNF-a). Thus, it was proved that CP antigens could be candidate for developing a vaccine against fascioliasis