MOLECULAR GENETIC STUDIES OF WHEAT RESISTANCE TO STEM RUST DISEASE
OMNIA OSAMA FOUAD MOHAMED;
Abstract
Wheat has been considered the first strategic food crop in Egypt.Wheat stem rust (caused by Pucciniagraminis f. sp. Tritici.) is among of the most serious diseases of wheat worldwide. It became more threatening after the emergence of the race Ug99 and its variants.
Resistant crops have been produced successfully for many years by conventional breeding techniques.Developing effective, durable resistant cultivars can be achieved when several stem rust resistance genes are pyramided into a single genotype which can be easily performed by using marker-assisted selection (MAS).
In this work, thirteen wheat cultivars; (Misr1, Misr2, Gemeza 10, Gemeza 11, Sids 1, Sids 12, Sids 13, Skha 93, CYMITT, Sahel1, Gemeza9, Gemeza7, and G168 were screened for the presence of stemrust resistance genes Sr2, Sr22, Sr24, Sr26, Sr33, Sr35, Sr36 and Sr40 using the simple sequence repeats (SSRs) molecular markers gwwm533, cfa2019, barc71, Sr26#43, abc156, cfa2193, gwm319, wmc477 and wmc661, the screening showed that most Egyptian cultivars have pyramided Sr genes as in Misr1 and CYMMITT which have four Srgenes, Gemiza7 and Sk93 have two Srgenes, while the rest have three Sr genes except G168 which has only one Sr gene. Then, the expression of the Sr35 gene was estimated in the cultivars Misr1, Misr2 and Gemiza10 which have this gene using real-time PCR, results showed that Gemiza10 has the highest expression level in response to stem rust infection followed by Misr1, while Misr2 has the lowest expression level of Sr35.
Resistant crops have been produced successfully for many years by conventional breeding techniques.Developing effective, durable resistant cultivars can be achieved when several stem rust resistance genes are pyramided into a single genotype which can be easily performed by using marker-assisted selection (MAS).
In this work, thirteen wheat cultivars; (Misr1, Misr2, Gemeza 10, Gemeza 11, Sids 1, Sids 12, Sids 13, Skha 93, CYMITT, Sahel1, Gemeza9, Gemeza7, and G168 were screened for the presence of stemrust resistance genes Sr2, Sr22, Sr24, Sr26, Sr33, Sr35, Sr36 and Sr40 using the simple sequence repeats (SSRs) molecular markers gwwm533, cfa2019, barc71, Sr26#43, abc156, cfa2193, gwm319, wmc477 and wmc661, the screening showed that most Egyptian cultivars have pyramided Sr genes as in Misr1 and CYMMITT which have four Srgenes, Gemiza7 and Sk93 have two Srgenes, while the rest have three Sr genes except G168 which has only one Sr gene. Then, the expression of the Sr35 gene was estimated in the cultivars Misr1, Misr2 and Gemiza10 which have this gene using real-time PCR, results showed that Gemiza10 has the highest expression level in response to stem rust infection followed by Misr1, while Misr2 has the lowest expression level of Sr35.
Other data
| Title | MOLECULAR GENETIC STUDIES OF WHEAT RESISTANCE TO STEM RUST DISEASE | Other Titles | دراسات وراثية جزيئية لمقاومة مرض صدأ الساق الأسود في القمح | Authors | OMNIA OSAMA FOUAD MOHAMED | Issue Date | 2016 |
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