Evaluation of Porous Titanium Granules As An Osteoconductive Material When Used In Endodontic Surgery (An in ViVo Study)

Abstract

The present study was performed to evaluate the biocompatibility of a new bone filling material "Tigran" and the healing potentiality of the osseous defects with the use of Porous titanium granules, after periapical surgery. Animal study: Twenty male albino rats weighing between 250-300 gm were chosen and classified equally into two main groups. In group (A) rats were examined and sacrificed one week after the operation, while in group (B) they were examined and sacrificed four weeks after the operation. All Rats were prepared, anesthetized and incised to expose the two femur bones. Bony defects were created in the right and left femur by using a sterile bur with a low speed hand piece, in which the right femur was filled with "Tigran" and the left one was free from "Tigran", Then suturing was done followed by swabbing the operating area using betadine to avoid surface contamination. Summary & Conclusion - 107 - Histopathological assessment : -Assessment of foreign body reaction: The "inflammatory cell count" was measured via an image analyser by using slides stained with H&E. -Bone deposition Assessment : Contrast between the collagen fibers in the newly formed woven bone and old bone was demonstrated in the defect area by using slides stained with Masson Trichrome stain. Human study: Twenty systemically healthy patients were selected and classified into two groups. Group (one), included ten patients who were treated by using "Tigran" after periapical surgery .While group (two), included ten patients who were treated by periapical surgery without "Tigran". Proper root canal treatment was done for all cases prior to the surgery. Following the aseptic conditions, patient preparation, administration of local anesthesia was done ,followed by choosing adequate flap design, incision, flap elevation and retraction, removal of the buccal bone of the offending tooth if present, excavation, curettage of the bony defect, apicectomy, and implantation of "Tigran" in the bony defects of group (one) while Summary & Conclusion - 108 - leaving it "Tigran-free" in group (two) followed by flap adaptation, repositioning, suturing and post operative patient instructions and medications to avoid infection..