The Genotoxic Effect Of Tetragonal Zirconia On Human Peripheral Blood Lymphocyte
Sarah Sayed Ahmed Hanafy;
Abstract
The aim of this study was to detect the genotoxicity of zirconia ceramics under different conditions non-glazed,glazed before and after aging on human peripheral blood lymphocytes.
Specimen of zirconia was prepared according to the manufacturer’s instructions aseptically into discs (machine cut from the zirconia blocks) of 5mm diameter and 2mm thickness and surface area of 75 mm2.
A total of 24 zirconia discs and 6 sample solution were tested for genotoxicity and were divided into three main groups;groupI(n=6) control groupcell-culture medium (RPMI 1640) incubated under same conditions (has no genotoxic effects) ,group II (n=12) non aged group with two subgroups one non glazed subgroup IIa(n=6) other is glazedsubgroupIIb(n=6),group III(n=12) aged group with two subgroups one non glazed subgroupIIIa(n=6) other is glazed subgroup IIIb (n=6).
In subgroup IIb andIIIb specimens were glazed using (VITA AKZENT glaze powder and fluid VITA (VT), Zahnfabrik H. Rauter GmbH & Co. KG) mixed tillcreamy mix is obtained applied with suitable brush in one brush strokes in one direction then fired in Programat P300 furnace IvoclarVivadent (IV),Schaan, FL.
In group III specimens were aged by Accelerated aging in a steam autoclave (M 11 ultraclave autoclave. Midmark USA. (MD)) for 8consecutive cycles in 134°C 2 barpressure.
Specimen of zirconia was prepared according to the manufacturer’s instructions aseptically into discs (machine cut from the zirconia blocks) of 5mm diameter and 2mm thickness and surface area of 75 mm2.
A total of 24 zirconia discs and 6 sample solution were tested for genotoxicity and were divided into three main groups;groupI(n=6) control groupcell-culture medium (RPMI 1640) incubated under same conditions (has no genotoxic effects) ,group II (n=12) non aged group with two subgroups one non glazed subgroup IIa(n=6) other is glazedsubgroupIIb(n=6),group III(n=12) aged group with two subgroups one non glazed subgroupIIIa(n=6) other is glazed subgroup IIIb (n=6).
In subgroup IIb andIIIb specimens were glazed using (VITA AKZENT glaze powder and fluid VITA (VT), Zahnfabrik H. Rauter GmbH & Co. KG) mixed tillcreamy mix is obtained applied with suitable brush in one brush strokes in one direction then fired in Programat P300 furnace IvoclarVivadent (IV),Schaan, FL.
In group III specimens were aged by Accelerated aging in a steam autoclave (M 11 ultraclave autoclave. Midmark USA. (MD)) for 8consecutive cycles in 134°C 2 barpressure.
Other data
| Title | The Genotoxic Effect Of Tetragonal Zirconia On Human Peripheral Blood Lymphocyte | Other Titles | آثار التسمم الجيني للزرقونيا رباعية الزوايا على الليمفويات البشرية | Authors | Sarah Sayed Ahmed Hanafy | Issue Date | 2015 |
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