EFFECT OF THE CARBAMATE "ALDICARB" ON ACETYL CHOLINESTERASE EXTRACTED FROM WHOLE AND DIFFERENT PARTS OF RAT BRAIN
Hassan Mohamed Yousry Osman;
Abstract
Aldicarb, a synthetic carbamate compound used as an insecticide and herbicide, is also employed as a pharmacological cholinergic agent in therapeutic forms.
Inhibition of the enzyme AChE is the basis for its use as an insecticide and it resulted in toxicity in a variety of animals including man. AChE is responsible for the metabolism of the neurochemical transmitter acetylcholine.
In this thesis, experiments were carried out in vitro and in vivo to study the effects of aldicarb on the activity of ChE enzyme extracted from whole and five different parts ofrat brain, namely: basal ganglia, frontal cmtex, medulla oblongata, pons and cerebellum. Kinetic measurements of Michaelis constants (Km), enzyme inhibitor dissociation constants (K,) as well as the rate of cerbamylation and binding constants for the inhibition of the enzyme obtained from the different parts, were done.
The results indicated that aldicarb which is a carbamate compound, had affected the activities of cholinesterase extracted from whole and five different parts of rat brain in vitro as well as in vivo.
The carbamylation rate (degree of inhibition of AChE by carbamate) and binding constant for inhibition of whole and different parts of brain in vitro, indicated that the carbamylation rate constants of pons, medulla oblongata and cerebellum were higher than those for other parts.
Inhibition of the enzyme AChE is the basis for its use as an insecticide and it resulted in toxicity in a variety of animals including man. AChE is responsible for the metabolism of the neurochemical transmitter acetylcholine.
In this thesis, experiments were carried out in vitro and in vivo to study the effects of aldicarb on the activity of ChE enzyme extracted from whole and five different parts ofrat brain, namely: basal ganglia, frontal cmtex, medulla oblongata, pons and cerebellum. Kinetic measurements of Michaelis constants (Km), enzyme inhibitor dissociation constants (K,) as well as the rate of cerbamylation and binding constants for the inhibition of the enzyme obtained from the different parts, were done.
The results indicated that aldicarb which is a carbamate compound, had affected the activities of cholinesterase extracted from whole and five different parts of rat brain in vitro as well as in vivo.
The carbamylation rate (degree of inhibition of AChE by carbamate) and binding constant for inhibition of whole and different parts of brain in vitro, indicated that the carbamylation rate constants of pons, medulla oblongata and cerebellum were higher than those for other parts.
Other data
| Title | EFFECT OF THE CARBAMATE "ALDICARB" ON ACETYL CHOLINESTERASE EXTRACTED FROM WHOLE AND DIFFERENT PARTS OF RAT BRAIN | Other Titles | تأثير الألديكارب الكارباميتى على إنزيم الأستيل كولين إستيريز المستخلص من بعض أجزاء المخ المختلفة فى الفئران | Authors | Hassan Mohamed Yousry Osman | Issue Date | 1999 |
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