MOLECULAR GENETIC STUDIES ON HEAT SHOCK RESPONSE IN DROSOPHILA
MARWA ROUSHDY SAYED MAHMOOD;
Abstract
Marwa Roushdy Sayed Mahmood. “Molecular Genetic Studies on Heat Shock Response in Drosophila”. Unpublished M.Sc. Thesis, Department of Genetics, Faculty of Agriculture, Ain Shams University, 2017.
The heat shock proteins (HSPs), abundantly expressed in insects, are important modulators of insect survival and are used as sensitive biomarkers for xenobiotics. Increased nanomaterial productions, including silver nanoparticles (AgNPs) and their wide range of applications imply a higher risk of human and environmental exposure.
The aims of the present study were to: 1. Investigate the impact of AgNPs exposure in D. melanogaster with regard to changes in the expression of heat shock (hsp) genes (hsp23, hsp26, hsp27, and hsp60). 2. Assess the correlations between hsps genes, antioxidant systems and oxidative stress as reflected by changes in the expression of antioxidant enzymes (SOD, CAT and GSH). 3. Assess the ability to tumors development by monitoring the changes in the expression of tumor suppressor gene (p53). 4. Detect some genetic biomarkers associated with biological stress in fruit flies.
Characterization of AgNPs by using transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis revealed agglomeration of silver particles in water, spherical shape, and uniform size with an average diameter of between 15-70 nm and the hydrodynamic diameters were 74.35 nm.
Larvae exposure to different concentrations of AgNPs resulted in significant changes in body color and some toxic effects such as melanization, necrosis and malformations and the larvae failed to pupate at higher concentrations. The acute toxic effect of AgNPs on D.melanogaster was observed at the AgNPs concentration of 1600 μg/ml. Silver concentration, resulted in 50% of the tested flies unable to leave the pupae, and they failed to complete their developmental cycle.
The heat shock proteins (HSPs), abundantly expressed in insects, are important modulators of insect survival and are used as sensitive biomarkers for xenobiotics. Increased nanomaterial productions, including silver nanoparticles (AgNPs) and their wide range of applications imply a higher risk of human and environmental exposure.
The aims of the present study were to: 1. Investigate the impact of AgNPs exposure in D. melanogaster with regard to changes in the expression of heat shock (hsp) genes (hsp23, hsp26, hsp27, and hsp60). 2. Assess the correlations between hsps genes, antioxidant systems and oxidative stress as reflected by changes in the expression of antioxidant enzymes (SOD, CAT and GSH). 3. Assess the ability to tumors development by monitoring the changes in the expression of tumor suppressor gene (p53). 4. Detect some genetic biomarkers associated with biological stress in fruit flies.
Characterization of AgNPs by using transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis revealed agglomeration of silver particles in water, spherical shape, and uniform size with an average diameter of between 15-70 nm and the hydrodynamic diameters were 74.35 nm.
Larvae exposure to different concentrations of AgNPs resulted in significant changes in body color and some toxic effects such as melanization, necrosis and malformations and the larvae failed to pupate at higher concentrations. The acute toxic effect of AgNPs on D.melanogaster was observed at the AgNPs concentration of 1600 μg/ml. Silver concentration, resulted in 50% of the tested flies unable to leave the pupae, and they failed to complete their developmental cycle.
Other data
| Title | MOLECULAR GENETIC STUDIES ON HEAT SHOCK RESPONSE IN DROSOPHILA | Other Titles | دراسات وراثية جزيئية على الاستجابة للصدمة الحرارية في الدروسوفيلا | Authors | MARWA ROUSHDY SAYED MAHMOOD | Issue Date | 2017 |
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