Biochemical Studies on Antioxidant Activity of Certain Irradiated Agro Industrial Wastes

Abstract

Food irradiation is a method of preservation; it is used to extend the shelf life of food products fresh and/or dried, destroy the contaminating harmful pathogens and modify the activity of bioactive compounds present in food materials. The present study was conducted to test the possible biochemical impacts of radiation processing on three agro industrial wastes (grape seed-skin, tomato pomace and artichoke leaves), as well as to elucidate the physiological and biochemical effects of feeding growing male Wister rats on diets supplemented with the processed agro industrial wastesat dose levels of 10, 20 and 30 kGy. The chemical composition of processed agro industrial wastes at the above mentioned doses showed no significant differences in corresponding to the non-irradiated one.The orthogonal statistical analysis of the data revealed thatthe linear, quadratic and/or cubic effects were not significantly different;however, an exception for these general observations was observed for fiber content where it was significantly reduced accompanied with increase in nitrogen free extract. Statistical analysis of polyphenol, tannin content and free radical scavenging activity results showed that irradiation processing caused an increase in polyphenols as a function of radiation dose, meanwhile grape seed-skin processed by 20 kGy gamma raysshoweda decrease by 15.38%, tannin content exhibited an increase in both grape seed-skin and artichoke leaves at all radiation doses used. In contrast, grape seed-skin irradiated at dose level 10 kGy has lost 25 % corresponding to their control one, same observation of reduction in tannins content of tomatopomace, was recorded. Regarding to the impact of irradiation treatment up to 30 kGy on free radical scavenging activity of grape seeds-skin, tomato pomaceand artichoke leaves, the results indicated that there was a significant reduction in their ability to scavenge free radicals, while an exception was observed for artichoke leaves processed by 10 kGy. The amino acid pattern was significantly affected by gamma irradiations, where some amino acids increased; othersdecreased,or did not change. It was observed that the changes in amino acids pattern as affected by irradiation up to and including 30 kGy was occurred without a specific trend in the three agro industrial wastes under investigation. The fatty acid profile of the three agro industrial wastes under investigation was significantly affected by radiation processing which caused an increase in some fatty acids, decrease in others and some did not change, compared to those corresponding fatty acids in non-irradiated three agro industrial wastes, with no specific trend. The physiological and biochemical performance of growing male Wisterrats as affected by feeding high fat diets supplemented with non-irradiated or irradiated up to and including 30 kGy agro industrial wastes under investigation for 8 weeks, showed better results when compared with those rats fed on reference diet. It has been manifested in body weight gain and internal organ weight alongside biochemicalaspects such as serum total cholesterol, triglycerides, LDL-C, HDL-C, VLDL-C, AI, ALT, AST and serum glucose.