TRANSFER OF PESTICIDE RESIDUES TO MICE OFFSPRING THROUGH PRENATAL AND LACTATION EXPOSURE
MARWA FAROUK AHMED MOHAMED GAD;
Abstract
This study evaluates toxicity of three pesticides [atrazine (A),
chlorpyrifos (C) and endosulfan (E)], and tertiary mixture (ACE) which
were mixed in the rodent diet at their respective “Acceptable Daily
Intake, ADI” (e.g., 31.0, 44.0 and 30.0 mg kg-1 /bw/day, respectively), in
addition to oral administration of vitamin E (α-tocopherol; 100 ul/
mouse). The experimental protocol included treatments during gestation
and lactation periods separately. Either during gestation or lactation, the
mouse dams were received one of the following treatments: (a) diet free
of pesticides; (b) diet enriched with one of the tested pesticides (c) diet
free of pesticides + oral vitamin E; and (d) diet enriched with one of the
tested pesticides + oral vitamin E. Drinking water was allowed ad libitum
up to 42 days. After the weaning, pups and dams were killed and selected
organs and blood samples were collected for analyses. Compared with the
control results either in dams or their pups, the tested pesticides induced
high elevation in AST, ALT, ALP, urea and MDA and high decline in
BuChE, SOD and CAT. Furthermore, the tested pesticides caused
histopathological damage in the internal organs (e.g., liver, kidney, ovary
and testis). The ameliorative effect of vitamin E, based on estimating the
“Amelioration Index; AI” revealed the powerful effect of this vitamin in
alleviating the oxidative stress exerted by the tested pesticides. The
findings of this study may support the need to further investigating the
adverse effects of exposure to low doses of commonly used pesticides,
especially during pregnancy and breast-feeding as well as effects on
newborn child.
Keywords: Atrazine; Chlorpyrifos; Endosulfan; Pesticide Mixture;
Vitamine E; Pregnant Mice ; Lactating Mice; Offspring;
Oxidative Stress
ACKNOWLEDGEMENT
All praises and thanks are due to ALLAH, who blessed me with kind
professors and colleagues, and gave me the support to complete this thesis.
First of all, i have to record my deep appreciation to late Professor Dr.
Zidan H. Zidan, Professor of Pesticide Chemistry and Toxicology, Faculty
of Agriculture, Ain-Shams University, who shared preparation of the
protocol of this thesis. I dedicate the results of this investigation to
chlorpyrifos (C) and endosulfan (E)], and tertiary mixture (ACE) which
were mixed in the rodent diet at their respective “Acceptable Daily
Intake, ADI” (e.g., 31.0, 44.0 and 30.0 mg kg-1 /bw/day, respectively), in
addition to oral administration of vitamin E (α-tocopherol; 100 ul/
mouse). The experimental protocol included treatments during gestation
and lactation periods separately. Either during gestation or lactation, the
mouse dams were received one of the following treatments: (a) diet free
of pesticides; (b) diet enriched with one of the tested pesticides (c) diet
free of pesticides + oral vitamin E; and (d) diet enriched with one of the
tested pesticides + oral vitamin E. Drinking water was allowed ad libitum
up to 42 days. After the weaning, pups and dams were killed and selected
organs and blood samples were collected for analyses. Compared with the
control results either in dams or their pups, the tested pesticides induced
high elevation in AST, ALT, ALP, urea and MDA and high decline in
BuChE, SOD and CAT. Furthermore, the tested pesticides caused
histopathological damage in the internal organs (e.g., liver, kidney, ovary
and testis). The ameliorative effect of vitamin E, based on estimating the
“Amelioration Index; AI” revealed the powerful effect of this vitamin in
alleviating the oxidative stress exerted by the tested pesticides. The
findings of this study may support the need to further investigating the
adverse effects of exposure to low doses of commonly used pesticides,
especially during pregnancy and breast-feeding as well as effects on
newborn child.
Keywords: Atrazine; Chlorpyrifos; Endosulfan; Pesticide Mixture;
Vitamine E; Pregnant Mice ; Lactating Mice; Offspring;
Oxidative Stress
ACKNOWLEDGEMENT
All praises and thanks are due to ALLAH, who blessed me with kind
professors and colleagues, and gave me the support to complete this thesis.
First of all, i have to record my deep appreciation to late Professor Dr.
Zidan H. Zidan, Professor of Pesticide Chemistry and Toxicology, Faculty
of Agriculture, Ain-Shams University, who shared preparation of the
protocol of this thesis. I dedicate the results of this investigation to
Other data
| Title | TRANSFER OF PESTICIDE RESIDUES TO MICE OFFSPRING THROUGH PRENATAL AND LACTATION EXPOSURE | Other Titles | إنتقال متبقيات المبيدات إلي ذرية الفئران عن طريق الأمهات أثناء فترة الحمل والرضاعة | Authors | MARWA FAROUK AHMED MOHAMED GAD | Issue Date | 2018 |
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