Phenotypic and genotypic characterization of Pseudomonas aeruginosa from different sources
Amal Ibrahem Attia Mansour;
Abstract
Abstract
In the present study the total number of P.aeruginosa isolated from 568 samples of animal and human origins represented as follows 450 bovine samples, 65 dog samples, and 53 samples of human origins, Positive isolates were 63 with an incidence of 11%. The P. aeruginosa recovery rate from milk samples of cows (8.4%.), from nasopharyngeal swabs of calves ( 9.5%) while, dog clinical samples (9.4%) and For human the recovery rate (18.8%) .The serogrouping reveled the most prevalent serogroupes O8 (50 %) , O6 (25%) On the other hand, serogroupes O3, O4, O5, O9 and O11 could not be recorded among bovine isolates. In dogs the serogroupe O4 was the most prevalent one (53.8%), & serogroupe O5 (46.2%). The human isolates revealed the most frequent serogroupes O8 (60%) & O6 (40%). The P. aeruginosa antimicrobial sensitivity were applied for all isolates which were variable in resistance patterns and most of isolates were MDR. The heat-shock gene detection by The PCR assay was applied and. all examined isolates are positive for groE gene confirming conventional biochemical characterization of isolates. Flagellin gene amplified product length 1020 bp separated in all but tested P. aeruginosa isolates. The molecular weight of tested 6 isolates revealed that protein molecular weight was varied in between 45 kDa lane 1.2.5 & 6. The other isolates (Lane 3 & 4) were produced molecular weight 50 kDa.
In the present study the total number of P.aeruginosa isolated from 568 samples of animal and human origins represented as follows 450 bovine samples, 65 dog samples, and 53 samples of human origins, Positive isolates were 63 with an incidence of 11%. The P. aeruginosa recovery rate from milk samples of cows (8.4%.), from nasopharyngeal swabs of calves ( 9.5%) while, dog clinical samples (9.4%) and For human the recovery rate (18.8%) .The serogrouping reveled the most prevalent serogroupes O8 (50 %) , O6 (25%) On the other hand, serogroupes O3, O4, O5, O9 and O11 could not be recorded among bovine isolates. In dogs the serogroupe O4 was the most prevalent one (53.8%), & serogroupe O5 (46.2%). The human isolates revealed the most frequent serogroupes O8 (60%) & O6 (40%). The P. aeruginosa antimicrobial sensitivity were applied for all isolates which were variable in resistance patterns and most of isolates were MDR. The heat-shock gene detection by The PCR assay was applied and. all examined isolates are positive for groE gene confirming conventional biochemical characterization of isolates. Flagellin gene amplified product length 1020 bp separated in all but tested P. aeruginosa isolates. The molecular weight of tested 6 isolates revealed that protein molecular weight was varied in between 45 kDa lane 1.2.5 & 6. The other isolates (Lane 3 & 4) were produced molecular weight 50 kDa.
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| Title | Phenotypic and genotypic characterization of Pseudomonas aeruginosa from different sources | Authors | Amal Ibrahem Attia Mansour | Issue Date | 2018 |
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