Isolation and characterization of mycoplasmas from wild and earthen pond raised fishes in Egypt

Abstract

1- Cyprinus carpio had the highest incidence of Mycoplasma isolation 5 out of 15 (33.33%), preceded by Oreochromis niloticus 29 (16.36%), then Aulopiformes Synodontida 6 (8.108%), and finally Clarias gariepinus 2 (6.45%). Mugil cephalus didn't show any incidence of Mycoplasma (0%). 2- The gills had high incidence of Mycoplasma isolation 42 (35.29%) and it is the only organ that the Mycoplasma was isolated from. 3- The incidence of Mycoplasma isolation was (20.16%) in Giza governorate, (15%) in Behira, Damietta (12.12%) and Port Said (2.43%). 4- PCR for the isolates using the universal primers for Mycoplasma gave similar results to that given by culture method Cyprinus carpio (33.33%), Oreochromis niloticus (16.36%), Aulopiformes Synodontida (8.108%), and finally Clarias gariepinus (6.45%). Mugil cephalus was negative by PCR for Mycoplasma. All examined samples were negative by PCR for Spiroplasma. 5- Mycoplasma recovered from gills was subjected to PCR using the Universal Mycoplasma primers which the same incidence (35.29%) as that recovered by culture. All examined samples were negative by PCR for Spiroplasma. 6- Mycoplasma was recovered from all examined governorates Giza (20.16%), Behira (15%), Damietta (12.12%), Kafr El Sheik