Efficacy of Phytochemical Extracts of Rosemary on Head and Neck Squamous Cell Carcinoma Cell LineYara Youssef Mouselhy Youssef
AbstractSCC is the most frequent malignant neoplasm affecting the head and neck region, and the incidence of SCC has been gradually increasing over the past few decades despite recent advances in the diagnosis and treatment(5). Recently, increasing interest in alternative PCD types, other than apoptosis, emerged once different tools to study these other genetically controlled systems at the molecular level became available(9). The status of autophagy in cancer therapy has also been given increasing attention(78). The dual role of autophagy in cancer is still controversial. Some studies indicated that malignancy is correlated with the suppression of autophagy, and the contradictory results have also been reported(150). Accumulating evidence shows that miRNA expression is de-regulated in many aspects of cell pathophysiology, including HNSCC(26). In principle, autophagy could be regulated by miRNAs targeting the mRNA of key molecules that indirectly induce or suppress autophagy(189). This study aimed to retrieve novel miRNA and selected autophagy target genes involved in HNSCC development and progression, and to characterize the efficacy of phytochemical extracts of rosemary on modulating miRNA-related HNSCC expression with its target ATG, utilizing HNSCC cell line. The study included nine groups, and the cell viability percentage for all groups was measured after 48 hours of treatment with different doses of the phytochemicals prepared according to the IC50 value. Real-time qPCR was performed for selected miRNA in this study, while cell viability
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