Potential Cardioprotective Effects of Vitamin D on Cardiotoxicity induced by Doxorubicin
Heba HossamEldin Ahmed Awad;
Abstract
DOX has a broad spectrum effect and high potency against wide range of human neoplasm. However, the clinical efficacy is limited by dose dependent cardiotoxicity. It was proved recently that Vit D plays an important role as anti-oxidant, anti apoptotic effect as well as its role in maintaining cardiovascular system functions. The present study was designed to investigate the potential protective effect of Vit D against DOX -induced cardiotoxcity in rats and to elucidate the possible underlying molecular mechanisms via studying its effects on different oxidative stress and apoptotic markers.
The present study aimed to screen the cardioprotective effect of Vit D against acute DOX cardiotoxicity.
Forty eight adult male rats weighing 120- 180gm were used in this study. Rats were kept in the animal house of the physiology department, faculty of medicine, Ain Shams University.
Rats were randomly assigned to four groups each group (n = 12):
Group I:Normal control rats.
Group II:Doxorobucin- treated rats.
Group III:Vit D pretreated - DOX - treated rats.
Group IV:Vit D treated rats.
The normal control ratsgroup Iwere received saline for the whole length of the protocol and considered as the control group. DOXgroup II (EBEWE Pharma Ges 50 mg/25ml) was used in a single intraperitoneal injection in a dose of 15 mg/kg. The rats of this group were sacrificed 24 hours following the injection. Rats of the group III, received a daily intraperitoneal injection of Vit D(Memphis for Pharmaceuticals & chemical industries)in a dose of 10,000 IU/100 g BW for a consecutive four days,in addition, in the forth day, the rats were received a single intraperitoneal injection of DOX in a dose of 15 mg/kg BW, one hour after the last dose of Vit D injection, and sacrificed after 24 hours. Rats of the group IV, received a daily intraperitoneal injection of Vit D for a consecutive four days in the same regimen as group III. On the day of sacrifice (24 hrs after DOX injection), both systolic and diastolic arterial blood pressure were measured for all rats. ECG recording was done under anaesthesia(thiopental sodium, 40 mg/kg B.W.i.p.). Blood samples were collected from the abdominal aorta and the plasma was separated and stored at - 80°c until used. Then, rats were sacrificed and heart tissue were dissected and washed with normal saline 0.9% and dried by filter paper. It was cleaned of the fat and fibrous tissue, and preserved at -80°c in parafilm for subsequent testing. In addition, heart specimens from different groups were fixed in 10% buffered formalin for histopathological and immunohistochemical.
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The present study aimed to screen the cardioprotective effect of Vit D against acute DOX cardiotoxicity.
Forty eight adult male rats weighing 120- 180gm were used in this study. Rats were kept in the animal house of the physiology department, faculty of medicine, Ain Shams University.
Rats were randomly assigned to four groups each group (n = 12):
Group I:Normal control rats.
Group II:Doxorobucin- treated rats.
Group III:Vit D pretreated - DOX - treated rats.
Group IV:Vit D treated rats.
The normal control ratsgroup Iwere received saline for the whole length of the protocol and considered as the control group. DOXgroup II (EBEWE Pharma Ges 50 mg/25ml) was used in a single intraperitoneal injection in a dose of 15 mg/kg. The rats of this group were sacrificed 24 hours following the injection. Rats of the group III, received a daily intraperitoneal injection of Vit D(Memphis for Pharmaceuticals & chemical industries)in a dose of 10,000 IU/100 g BW for a consecutive four days,in addition, in the forth day, the rats were received a single intraperitoneal injection of DOX in a dose of 15 mg/kg BW, one hour after the last dose of Vit D injection, and sacrificed after 24 hours. Rats of the group IV, received a daily intraperitoneal injection of Vit D for a consecutive four days in the same regimen as group III. On the day of sacrifice (24 hrs after DOX injection), both systolic and diastolic arterial blood pressure were measured for all rats. ECG recording was done under anaesthesia(thiopental sodium, 40 mg/kg B.W.i.p.). Blood samples were collected from the abdominal aorta and the plasma was separated and stored at - 80°c until used. Then, rats were sacrificed and heart tissue were dissected and washed with normal saline 0.9% and dried by filter paper. It was cleaned of the fat and fibrous tissue, and preserved at -80°c in parafilm for subsequent testing. In addition, heart specimens from different groups were fixed in 10% buffered formalin for histopathological and immunohistochemical.
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Other data
| Title | Potential Cardioprotective Effects of Vitamin D on Cardiotoxicity induced by Doxorubicin | Other Titles | التأثيرالوقائي المحتمللفيتامين Dفى سمية القلب المحدثة بدواء دكسوروبسين فى الجرذان | Authors | Heba HossamEldin Ahmed Awad | Issue Date | 2015 |
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