Impact of some environmental conditions on RuBisCO enzyme
Ayat Salah Sakran;
Abstract
The present study was carried out during the period from 2013 to 2016 at Botany Department, Faculty of Science, Ain Shams University (ASU).
Abiotic stresses are the main factors leading to crop loss. Universally, more than 50 % of major crops are lost solely due to this reason. While, the pea crop considered as one of the most important annual food crops that have many desired characters and neumerous benefits. Therefore, this study was carried out for monitoring the effect of some abiotic conditions on total cellular and chloroplast protein banding profiles using SDS-PAGE technique. In addition, study the impact of these conditions on the assembly of RuBisCO complex by using HDN-PAGE technique and definition of some detected protein bands using nano HPLC-ESI-MS/MS. Plants were treated as following:
1. Salinity treatment: Pea seedlings were transferred after three weeks from MS germination media to MS media supplemented with 50, 100, 150, 200, 300 and 400 mM of NaCl for 2, 3, 5 and 7 days. One set of plants kept on MS media free from NaCl as control.
2. Temperature treatment: Pea seedlings were transferred after two weeks from 20 °C growth room to 6,10 °C (for chilling stress) and 30, 40 °C (for heat stress) in light for 30 min, 1 and 2 hrs. One set of plants was kept at 20 °C a control.
3. Jasmonic Acid (JA) treatment: Pea seedlings were subjected to JA through application of three different strategies:
4. Strategy I: Pea seedlings were transferred from MS germination media to MS media with 0.5, 5 and 10 µM of JA for 1, 3 and 5 days. One set of plants were kept on MS media free from JA as control.
5. Strategy II: Sets of sterilized seeds were soaked in 1 µM jasmonic acid for 4, 8 and 12 hrs and plated on 0.8% agar media and left for germination for 3 weeks then collected for protein analysis. One set of seeds were plated on MS media without soaking as control.
6. Strategy III: Sets of sterilized seeds were plated on 0.8% agar media containing 0.5 and 10 µM of JA and left for germination for 3 weeks then seedlings were collected for protein analysis. One set of plants were kept on MS media free from JA as control.
Abiotic stresses are the main factors leading to crop loss. Universally, more than 50 % of major crops are lost solely due to this reason. While, the pea crop considered as one of the most important annual food crops that have many desired characters and neumerous benefits. Therefore, this study was carried out for monitoring the effect of some abiotic conditions on total cellular and chloroplast protein banding profiles using SDS-PAGE technique. In addition, study the impact of these conditions on the assembly of RuBisCO complex by using HDN-PAGE technique and definition of some detected protein bands using nano HPLC-ESI-MS/MS. Plants were treated as following:
1. Salinity treatment: Pea seedlings were transferred after three weeks from MS germination media to MS media supplemented with 50, 100, 150, 200, 300 and 400 mM of NaCl for 2, 3, 5 and 7 days. One set of plants kept on MS media free from NaCl as control.
2. Temperature treatment: Pea seedlings were transferred after two weeks from 20 °C growth room to 6,10 °C (for chilling stress) and 30, 40 °C (for heat stress) in light for 30 min, 1 and 2 hrs. One set of plants was kept at 20 °C a control.
3. Jasmonic Acid (JA) treatment: Pea seedlings were subjected to JA through application of three different strategies:
4. Strategy I: Pea seedlings were transferred from MS germination media to MS media with 0.5, 5 and 10 µM of JA for 1, 3 and 5 days. One set of plants were kept on MS media free from JA as control.
5. Strategy II: Sets of sterilized seeds were soaked in 1 µM jasmonic acid for 4, 8 and 12 hrs and plated on 0.8% agar media and left for germination for 3 weeks then collected for protein analysis. One set of seeds were plated on MS media without soaking as control.
6. Strategy III: Sets of sterilized seeds were plated on 0.8% agar media containing 0.5 and 10 µM of JA and left for germination for 3 weeks then seedlings were collected for protein analysis. One set of plants were kept on MS media free from JA as control.
Other data
| Title | Impact of some environmental conditions on RuBisCO enzyme | Other Titles | تأثير بعض الظروف البيئية على إنزيم الروبيسكو (RuBisCO) | Authors | Ayat Salah Sakran | Issue Date | 2016 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| G11680.pdf | 562.8 kB | Adobe PDF | View/Open |
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