Effect of Some Plants on Glucose Level and Insulin Blood in Induced Diabetic Rats Using Nanotechnology

Abstract

The present study was applied in Faculty of Specific Education Chemistry and Nutrition Laboratories, Ain Shams Univ.to investigate the effect of some plants on glucose and insulin in induced diabetic rats using nanotechnology. Fenugreek seeds was purchased from The local market, washed, dried then roasted at temperature (70°C) and grounded and reserved in plastic bag, Organic solvents, Chemical reagents, Silver Nitrate (AgNO3) and antidiabetic medication (Amyral) were purchased from Cairo, Egypt. Fenugreek seeds were extracted in Soxhlet apparatus for 8h/day for 3consecutive days. The extract was filtered and stored at 4°C. The chemical composition of Fenugreek extract was analyzed by (Infra-red), then synthesis of silver –nanoparticles (AgNPs) finally, a solution of fenugreek extract was mixed with AgNPs soulation to form residue products; Fenugreek –AgNPs.Female albino rats Sprague Dawaly (56rats) weighing approximately (180±20g) were used.They were purachased from Helwan breeding farm, Cairo, Egypt. The groups (2, 4, 6, and 7) were induced by injection with streptozotocin at (45 mg/kg body wt.) were divided 7 groups subgroups (8 rats each), all groups fed 2 months:

•Groups (1, 2): Normal, Diabetic rats: fed on basal diet only (no intervation). •Groups (3, 4): Normal, Diabetic rats: fed on basal diet+ Ag-NPs (200mg/ Kg b.w. /day) via oral gavage. •Groups (5, 6): Normal, Diabeticrats: fed on basal diet+ Fenugreek-Ag nanoparticles (200mg/ Kg b.w. /day) via oral gavage. •Group(7): Diabetic rats: fed on basal diet+ anti diabetic medication (300mg/Kg.b.wt/day) via oral gavage. At the end of the experiment (2 months).FI, BWG, and FER weekly.Rats were fasted overnight 12 hrs, anesthetized and blood samples were taken for the biochemical analysis, internal organs weighted and the relative weight.Data obtained werestatistically analyazed. The results showed that: •BWG and FER on diabetic G6 given Fenugreek-Agnanoparticles Oral administration were significantly increased (68.47g) and (1.27%). • Serum glucose, HbA1C on diabetic rats (G4, G6 and G7) given Fenugreek- Silver nanoparticles (F-AgNPs), Silver nanoparticles(AgNPS) and antidiabetic medication (Amyral) Oral administration were significantly decreased (247.98, 217.62 and 190.02) mg/dl and (7.49, 7.02 and 3.61) % respectively. In the G4 treatment with silver nanoparticles was significantly increased insulin (9.04 U/L) compared with diabetic G2 fed on basal diet only. • That significantly serum Total cholesterol and TG on diabetic rats (G4, G6 and G7) given Silver nanoparticles(AgNPS), Fenugreek +Silver nanoparticles (F-AgNPs),and antidiabetic medicationoral administration (75.25, 79.75,and 69.38)mg/dl and (85.59, 124.9 and 102.48)mg/dl respectively compared diabetic G2 fed on basal diet only. In the time normal groups non significantly compared G1 fed on basal diet only. •The effects of on the liver functions Fenugreek Silver nanoparticles (F-AgNPs), Silver nanoparticles (AgNPS) and Amyral (antidiabetic medication) Oral administration GPT and GOT were reduce a significant in diabetic groups (G4, G6 and G7) compared diabetic G2 fed on basal diet only on the other hand, total protein results showed a significant enhancement in diabetic groups (G4, G6, and G7) compared diabetic G2 fed on basal diet only. • Resulted proved that effect Fenugreek+ Silver nanoparticles (F-AgNPs), Silver nanoparticles (AgNPS) and antidiabetic medication oral administration on kidney functions in groups