The Ameliorative Effect of Propolis Extract Against Methotrexate Induced Oxidative Damage In Rats
Nourhan Gamal Mohamed Abd EL-Magid EL-Rahmany;
Abstract
Methotrexate (MTX), a folic acid antagonist, is commonly administered as a cytotoxic chemotherapeutic drug for various diseases (auto-immune diseases and various types of cancer). It has the potential to induce oxidative stress especially in the presence of insufficient endogenous antioxidant enzymes within the biological system. Patients taking methotrexate are more likely to discontinue therapy because of the adverse effects of medication.
Apitherapy or therapy with bee products (e.g. honey, pollen, propolis and fortified honey) is an old tradition that has been revived by recent researchers. These products, which are used as health foods and medicines are receiving renewed focus on their beneficial effects in a general “back to nature” trend.
The present study was conducted to investigate ameliorative effect of propolis against methotrexate induced oxidative damage on blood and liver, kidney and brain tissues in rats.
Analysis of the nutritive value showed that each 100 g of propolis sample contains 4 g protein, 2.11 g fat, 66.24 g carbohydrate, 16.65 g fiber, 10.5 g ash, 0.5g moisture, 1.98 mg iron, 15.93 mg calcium and 20.3 mg sodium. Phytochemical and antioxidant analysis data showed that 100 ml of propolis contains 1122.4 mg gallic acid and 2885 mg catechin which are equivalent to total phenols and flavonoids respectively. In addition, the results showed highly total antioxidant capacity for propolis which represented as 178 mg ascorbic acid. While gas chromatograpy-mass spectrometry (GC-MS) screening of propolis sample revealed the presence of caffeic acid phenethyl ester, chrysin, galangin, quercetin, kaempferol, pinocembrin and tectochrysin.
A total number of one hundred twenty healthy male Wistar albino rats, weighing 90g ± 5g were subjected to experimentation. Animals were assigned to one of 5 groups and administered their respective doses for 3, 6 and 9 weeks’ time intervals. The groups were divided as the following:
Group 1 (healthy control group): Rats fed on the standard commercial diet.
Group 2 (Saline + DMSO group): Rats fed on the standard commercial diet, injected with saline (i.p.) and administered DMSO (p.o.).
Group 3 (PP group): Rats fed on the standard commercial diet and treated with propolis (200 mg/kg b.wt. twice/week) (p.o.).
Group 4 (MTX group): Rats fed on the standard commercial diet and injected with methotrexate (2.5 mg/kg b.wt. single dose/week) (i.p.).
Group 5 (MTX + PP group): Rats fed on the standard commercial diet, injected with methotrexate (2.5 mg/kg b.wt. single dose/week) (i.p.) and administered propolis (200 mg/kg b.wt. twice/week) (p.o.).
Clinical hematological variables (hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, red blood cell count, white blood cell count, lymphocyte % and platelets count) were estimat
Apitherapy or therapy with bee products (e.g. honey, pollen, propolis and fortified honey) is an old tradition that has been revived by recent researchers. These products, which are used as health foods and medicines are receiving renewed focus on their beneficial effects in a general “back to nature” trend.
The present study was conducted to investigate ameliorative effect of propolis against methotrexate induced oxidative damage on blood and liver, kidney and brain tissues in rats.
Analysis of the nutritive value showed that each 100 g of propolis sample contains 4 g protein, 2.11 g fat, 66.24 g carbohydrate, 16.65 g fiber, 10.5 g ash, 0.5g moisture, 1.98 mg iron, 15.93 mg calcium and 20.3 mg sodium. Phytochemical and antioxidant analysis data showed that 100 ml of propolis contains 1122.4 mg gallic acid and 2885 mg catechin which are equivalent to total phenols and flavonoids respectively. In addition, the results showed highly total antioxidant capacity for propolis which represented as 178 mg ascorbic acid. While gas chromatograpy-mass spectrometry (GC-MS) screening of propolis sample revealed the presence of caffeic acid phenethyl ester, chrysin, galangin, quercetin, kaempferol, pinocembrin and tectochrysin.
A total number of one hundred twenty healthy male Wistar albino rats, weighing 90g ± 5g were subjected to experimentation. Animals were assigned to one of 5 groups and administered their respective doses for 3, 6 and 9 weeks’ time intervals. The groups were divided as the following:
Group 1 (healthy control group): Rats fed on the standard commercial diet.
Group 2 (Saline + DMSO group): Rats fed on the standard commercial diet, injected with saline (i.p.) and administered DMSO (p.o.).
Group 3 (PP group): Rats fed on the standard commercial diet and treated with propolis (200 mg/kg b.wt. twice/week) (p.o.).
Group 4 (MTX group): Rats fed on the standard commercial diet and injected with methotrexate (2.5 mg/kg b.wt. single dose/week) (i.p.).
Group 5 (MTX + PP group): Rats fed on the standard commercial diet, injected with methotrexate (2.5 mg/kg b.wt. single dose/week) (i.p.) and administered propolis (200 mg/kg b.wt. twice/week) (p.o.).
Clinical hematological variables (hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, red blood cell count, white blood cell count, lymphocyte % and platelets count) were estimat
Other data
| Title | The Ameliorative Effect of Propolis Extract Against Methotrexate Induced Oxidative Damage In Rats | Other Titles | التأثير المحسن لمستخلص صمغ النحل ضد التلف التأكسدى المحدث بواسطة الميثوتركسيت في الجرذان | Authors | Nourhan Gamal Mohamed Abd EL-Magid EL-Rahmany | Issue Date | 2015 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| G12140.pdf | 993.06 kB | Adobe PDF | View/Open |
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