PHYSIOLOGICAL STUDIES ON THE APPLICATION OF SOME PESTICIDES TO SOYBEAN PLANT (Glycine max L.) IN RELATION TO THE USE OF TISSUE CULTURE TECHNIQUE

IBRAHIM SElFEL-DIN IBRAHIM ALY


Abstract


The present investigation aimed to study the hazard effects of using some pesticides on plant physiology and biochemistry as well as plant anatomy in a tolerant plant species soybean. A number of different pesticides, i.e., Benlate (Fungicide), Fusilade (Herbicide) and Actellic (Insecticide) were applied to test plant at different concentrations and variable dates of applications.. Two experiments were conducted in 1993-1994 in the Experimental Laboratory and in the Field of the Fac. of Agric., Ain-Shams Univ., at Shoubra EI-Kheima Cairo, Egypt. Samples were taken for histological, physiological and biochemical studies in the Plant Biology Dept. at Univ. of Maryland College Park (UMCP) U.S. The results could be summarized in the following: 1. The results of soybean suspension cultures were more conceivable because the dose of pesticides directly reached the cells and was absorbed at a high •level. On the other hand, the results of the field experiment were less noticeable because of: a(The intact higher plant was more resistant to pesticide absorption, b(The dose reaching the cells was quite low, and c(the differences in time of application and sampling. 2. Benlate treatments did not affect fresh and dry weights. Fusilade treatments (F, 10%F) caused a decrease in fresh and dry weights while treatment (1%F) gave no effect. Actellic treatments led to similar fresh and dry weights that were noticed for Fusilade treatments. 3. Histologically, Benlate (B) led to differences in cell shape and size, with a thick cell wall and suffer mitotic abnormalities. Fusilade (F) and AC:tellic (A) treatments led to highly wrinkled, misshapen, large and less divided cells. Actellic treatment (1%A) was of low effect on cells than treatment (A).


Other data

Other Titles دراسات فسيولوجية على استخدام بعض مبيدات الافات على نبات فول الصويا بالاستعانة بتكنولوجيا زراعة الانسجة
Issue Date 1996
URI http://research.asu.edu.eg/handle/12345678/2699


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