AVOID OF BACTERIAL CONTAMINANTS IN TISSUE CULTURES
AMANY ELIWAY ALISAID;
Abstract
This research was designed to study the biological effects of actinomycetes and essential oils on microbial contaminants in banana plants tissue culture in vitro. Microbial contaminations are considered to be of the most serious constraints facing banana plant in tissue culture proliferation in vitro.Four antibiotics, namely rifampicin, gentamicin, chloramphenicol and vancomycin each at 50, 100 and 200 mg /litre and three antifungal agents, namely ketoconazole, fluconazole and nystatin each at 50, 100 and 200 mg/litre were used in the culture susceptibility tests of the identified bacteria and fungi, respectively.
To control banana plants contaminated by bacteria in vitro, actinomycetes (Streptomyces bobilii or S. grisiobrunneus) were tested. Actinomycetes as antimicrobial agent were tested in two ways, first in Petri dishes to test the antagonism between antimicrobial agents from actinomycetes and bacteria as well as fungi contamination, second in culture medium from the beginning at concentration of ( 0, 5, 10 and 15%) in different stages of banana indirect stages (shooting and rooting). Data obtained showed that, after twelve weeks, S. grisiobrunneus was more effective than S. bobilii at any concentration tested and at different stages. Addition of S. grisiobrunneus at the concentration of 10 % to the culture media during all studied stages was the prefer treatment which caused the best number of explants free contamination and the best number of survived explants.
Moreover, seven commercial pure essential oils were tested as antimicrobial activities i.e. Peppermint Oil, Coriander Oil, Ginger Oil, Marjoram Oil, Nigella Sativa Oil, Onion Oil, and Garlic Oil as 1.0 cm oil per 50 ml Murashige and Skoog (MS) medium before solidified medium. Media supplemented with essential oils, were compared to control medium, autoclaved at 121°C for 20 min. From our results we found that, Coriander Oil was the best in controlling the contamination and also more healthy on plant. The ratio of contamination after addition of coriander oil on Murashige and Skoog (MS) medium was reached to 33.3%. While, the other best oil used was Nigella sativa which gave a contamination reached to 41.6% and also healthier plant.
Key words: Antibiotic treatment, Antifungal treatment, Actinomycetes, Bacteria, Fungi, Essential oil, Banana, Contaminants and MS medium
To control banana plants contaminated by bacteria in vitro, actinomycetes (Streptomyces bobilii or S. grisiobrunneus) were tested. Actinomycetes as antimicrobial agent were tested in two ways, first in Petri dishes to test the antagonism between antimicrobial agents from actinomycetes and bacteria as well as fungi contamination, second in culture medium from the beginning at concentration of ( 0, 5, 10 and 15%) in different stages of banana indirect stages (shooting and rooting). Data obtained showed that, after twelve weeks, S. grisiobrunneus was more effective than S. bobilii at any concentration tested and at different stages. Addition of S. grisiobrunneus at the concentration of 10 % to the culture media during all studied stages was the prefer treatment which caused the best number of explants free contamination and the best number of survived explants.
Moreover, seven commercial pure essential oils were tested as antimicrobial activities i.e. Peppermint Oil, Coriander Oil, Ginger Oil, Marjoram Oil, Nigella Sativa Oil, Onion Oil, and Garlic Oil as 1.0 cm oil per 50 ml Murashige and Skoog (MS) medium before solidified medium. Media supplemented with essential oils, were compared to control medium, autoclaved at 121°C for 20 min. From our results we found that, Coriander Oil was the best in controlling the contamination and also more healthy on plant. The ratio of contamination after addition of coriander oil on Murashige and Skoog (MS) medium was reached to 33.3%. While, the other best oil used was Nigella sativa which gave a contamination reached to 41.6% and also healthier plant.
Key words: Antibiotic treatment, Antifungal treatment, Actinomycetes, Bacteria, Fungi, Essential oil, Banana, Contaminants and MS medium
Other data
| Title | AVOID OF BACTERIAL CONTAMINANTS IN TISSUE CULTURES | Other Titles | التخلص منالملوثات البكتيرية في مزارع الأنسجة | Authors | AMANY ELIWAY ALISAID | Issue Date | 2017 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.