A PILOT STUDY ON THE PREVALENCE OF GAD 65 AND IA-2 ANTIBODIES IN INSULIN DEPENDENT DIABETIC PATIENTS
Nagwa Abd EI-Hamid Arafa;
Abstract
The study was conducted on 51 newly diagnosed type I diabetic children with a mean age of 8.57±4.02 ranging from 1.4 to 15.0 years with a male/female ratio (31/20).
All cases were recruited from those attending the diabetic endocrine metabolic pediatric unit (DEMPU) children Hospital, Cairo University.
Patients were divided into 3 groups according to age of diabetes onset (< 5 years, 5-l 0 years and 10-15 years) and 3 groups according to duration of the disease (< 2 months, 2-6 months and > 6 months).
47 age and sex matched control subjects were included. Screening for autoantibodies for type I diabetes was performed:
- Islet cell antibody (ICA) by ELISA.
- Glutamic acid decarboxylase 65 antibody (GAD65A) by RIA.
- Tyrosine phosphatase antibody (IA-2A) by RIA.
the present study demonstrates that GAD65As assays are more frequently encounterd in type I diabetic patients than IA-2A and ICA values being 62.7%, 36.2% and 17.6% respectively and the prevalence of any one of the three autoantibody was 76.4% which was higher frequency than any of antibody alone. However the diagnostic sensitivity of the various antibody assays investigated tends to vary according to age at diabetes onset, both GAD65A and IA-2A being relatively higher at 5-l 0 years and in addition they are encountered more frequently in the group with diabetes duration of 2-6 months while ICA antibodies were less frequently encountered at these duration and age groups.
All cases were recruited from those attending the diabetic endocrine metabolic pediatric unit (DEMPU) children Hospital, Cairo University.
Patients were divided into 3 groups according to age of diabetes onset (< 5 years, 5-l 0 years and 10-15 years) and 3 groups according to duration of the disease (< 2 months, 2-6 months and > 6 months).
47 age and sex matched control subjects were included. Screening for autoantibodies for type I diabetes was performed:
- Islet cell antibody (ICA) by ELISA.
- Glutamic acid decarboxylase 65 antibody (GAD65A) by RIA.
- Tyrosine phosphatase antibody (IA-2A) by RIA.
the present study demonstrates that GAD65As assays are more frequently encounterd in type I diabetic patients than IA-2A and ICA values being 62.7%, 36.2% and 17.6% respectively and the prevalence of any one of the three autoantibody was 76.4% which was higher frequency than any of antibody alone. However the diagnostic sensitivity of the various antibody assays investigated tends to vary according to age at diabetes onset, both GAD65A and IA-2A being relatively higher at 5-l 0 years and in addition they are encountered more frequently in the group with diabetes duration of 2-6 months while ICA antibodies were less frequently encountered at these duration and age groups.
Other data
| Title | A PILOT STUDY ON THE PREVALENCE OF GAD 65 AND IA-2 ANTIBODIES IN INSULIN DEPENDENT DIABETIC PATIENTS | Other Titles | الدراسة الاسترشادية لنسبة وجود الأجسام المضادة للـ ج.أ.د. 65 (gad 65 ) وأى ايه 2 ( IA -2 ) فى حالات مرضى السكر معتمد على الانسولين | Authors | Nagwa Abd EI-Hamid Arafa | Issue Date | 2001 |
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