Phytochemical and Biological Studies of Caesalpinia gilliesii (Family Caesalpinacae) Growing in Egypt.

Abstract

Both in-vitro (antioxidant as well as antimicrobial, quorum sensing & cytotoxic) and in-vivo (anti-inflammatory, gastro ulcer, & hepatoprotective) bioassays guided fractionation of hydroalcoholic (70%) extract of Caesalpinia gilliesii (Hook) leaves and its fractions were studied. Phytochemical studies afforded a new polyoxygenated flavonol robionobioside (gossypetin-3-O-robinobioside) beside thirteen known compounds from the bioactive butanol fraction. The structures of these isolated compounds were elucidated on the basis of detailed (1D and 2D) NMR spectroscopic analysis and mass spectrometry. Quercetin showed potent quorum sensing activity at 15 and 75 µg/ml. Green synthesis of Ag NPs using C. gilliesii leaves extract showed a potent antimicrobial agents with a promising cytotoxic activity against normal skin cell line and breast cancer cell line. Keywords: Phytochemical & Biological studies, Caesalpinia gilliesii, Family Caesalpinacae, antioxidant, antimicrobial, Quorum sensing, cytotoxicity, Anti-inflammatory, Gastro ulcer, Hepato-protective, AgNPs, Green synthesis, In-vivo and In-vitro bioass