BIOCHEMICAL STUDIES ON HEPARIN •ISOLATED FROM INTESTINAL MUCOSA
Mohamed Mahmoud Usama Nooman;
Abstract
Heparin was isolated from bovine intestinal mucosa by using two methods, the first was sodium sulfate, sodium acetate and zinc oxide method and the second was ammonium sulfate method. By repeating the two methods several times and purification of the resulting heparin by potassium permanganate, it was found that 1 kg of bovine intestinal mucosa grves an average yield of 55 mg of purified heparin.
Chemical analysis of the prepared heparin samples revealed that there was a non significant difference in the percentages of glucuronic acid, sulfate ions and glucosamine between the prepared heparin samples and the standard heparin obtained from BIOFAC company.
Chemical analysis of the prepared heparin samples revealed that there was a non significant difference in the percentages of glucuronic acid, sulfate ions and glucosamine between the prepared heparin samples and the standard heparin obtained from BIOFAC company.
Other data
| Title | BIOCHEMICAL STUDIES ON HEPARIN •ISOLATED FROM INTESTINAL MUCOSA | Other Titles | دراسة بيوكيميائية على الهيبارين المفصول من الغشاء المخاطى للامعاء الدقيقة | Authors | Mohamed Mahmoud Usama Nooman | Issue Date | 1998 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| محمد محمود اسامة.pdf | 1.23 MB | Adobe PDF | View/Open |
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