Hebat Allah Ibrahim Mohamed
Hebat Allah Ibrahim Mohamed;
Abstract
The present study was planned to evaluate the effect of Withania somnifera extract / Gadolinium III oxide nanocomposite (WSGNC) as radiosensitizer and anticancer in tumour model of animals which leads to reduce the treatment dose of radiation during radiotherapy.
Cell viability was assayed in vitro and in vivo studies, tumour size and weight were also determined. DNA fragmentation, caspase-3 activity, mitochondrial enzymes activities, NADH-ubiquinone oxidodreductase (complex I), NADH-cytochrome c oxidoreductase (Complex II) and succinate-cytochrome c oxidoreductase (Complex III) were determined in cancer tissues. In addition oxidative stress was assayed by measuring the content of malondialdehyde (MDA) as a marker of lipid peroxidation. Glutathione content (GSH) as well as superoxide dismutase (SOD) and catalase activities were determined as markers of the antioxidant status.
The present study was carried out on 190 female albino mice body weight 22-25g and solid tumours were produced in 130 mice by intramuscular inoculation with 0.2 ml of EAC, which contained 2.5 x 106 viable EAC cells, in the right thigh of the lower limb of each mouse. Mice with a palpable solid tumour diameter (10mm³) that developed within 10 days after inoculation were used in the study.
Experimentl I:
In the present study animals were divided into seven main groups, each group contains 10 mice:
Group I: Animals bearing tumour left without any treatment.
Group II: Animals bearing tumour were exposed to 3 doses of
radiation (2 Gy / dose) weekly for 3 weeks.
Group III: Animals bearing tumour were injected i.p with
(WSGNC) (3 times / week) in a dose of 227 mg/kg b.w then exposed to 3 doses of radiation (2 Gy / dose) weekly for 3 weeks.
Group IV: Animals bearing tumour were exposed to 3 doses of
radiation (4 Gy / dose) weekly for 3 weeks.
Group V: Animals bearing tumour were injected i.p with
(WSGNC) (3 times / week) in a dose of 227 mg/kg b.w then exposed to 3 doses of radiation (4 Gy / dose) weekly for 3 weeks.
Group VI: Animals bearing tumour were exposed to 3 doses of
radiation (6 Gy / dose) weekly for 3 weeks.
Group VII: Animals bearing tumour were injected i.p with
Cell viability was assayed in vitro and in vivo studies, tumour size and weight were also determined. DNA fragmentation, caspase-3 activity, mitochondrial enzymes activities, NADH-ubiquinone oxidodreductase (complex I), NADH-cytochrome c oxidoreductase (Complex II) and succinate-cytochrome c oxidoreductase (Complex III) were determined in cancer tissues. In addition oxidative stress was assayed by measuring the content of malondialdehyde (MDA) as a marker of lipid peroxidation. Glutathione content (GSH) as well as superoxide dismutase (SOD) and catalase activities were determined as markers of the antioxidant status.
The present study was carried out on 190 female albino mice body weight 22-25g and solid tumours were produced in 130 mice by intramuscular inoculation with 0.2 ml of EAC, which contained 2.5 x 106 viable EAC cells, in the right thigh of the lower limb of each mouse. Mice with a palpable solid tumour diameter (10mm³) that developed within 10 days after inoculation were used in the study.
Experimentl I:
In the present study animals were divided into seven main groups, each group contains 10 mice:
Group I: Animals bearing tumour left without any treatment.
Group II: Animals bearing tumour were exposed to 3 doses of
radiation (2 Gy / dose) weekly for 3 weeks.
Group III: Animals bearing tumour were injected i.p with
(WSGNC) (3 times / week) in a dose of 227 mg/kg b.w then exposed to 3 doses of radiation (2 Gy / dose) weekly for 3 weeks.
Group IV: Animals bearing tumour were exposed to 3 doses of
radiation (4 Gy / dose) weekly for 3 weeks.
Group V: Animals bearing tumour were injected i.p with
(WSGNC) (3 times / week) in a dose of 227 mg/kg b.w then exposed to 3 doses of radiation (4 Gy / dose) weekly for 3 weeks.
Group VI: Animals bearing tumour were exposed to 3 doses of
radiation (6 Gy / dose) weekly for 3 weeks.
Group VII: Animals bearing tumour were injected i.p with
Other data
| Title | Hebat Allah Ibrahim Mohamed | Other Titles | التأثيرالمضاد للأورام والمنشط للإشعاع لمركب النانومع مستخلص نبات الأشواجندا فى الفئران المحملة بخلايا سرطانية | Authors | Hebat Allah Ibrahim Mohamed | Issue Date | 2016 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| G13627.pdf | 261.2 kB | Adobe PDF | View/Open |
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