Study on the Effects of Pre-existing Hyperuricemia and its Possible Pro-oxidant and Antioxidant Role on Renal Ischemia-reperfusion Injury in Male Albino Rats
Elsayed Ghonamy Mahros Ismail;
Abstract
The present study was planned in order to assess effect of preexisting hyperuricemia, regarding its pro oxidant or its antioxidant effect, in aggravating or ameliorating the outcome from the later on exposure to renal ischemia reperfusion injury.
This work was performed on adultmale Wistar rats, dividedinto 4 groups:
Group I:Sham control rats.
Group II: Hyperurecemic rats. Hyperurecemia was induced subcutaneous injection ofOxonic acid (2%) in a dose of 100 mg /Kg/day for 6 days for two weeks.
Group III: Renal Ischemic- reperfused rats (renal I/R).
Group IV: 2 weeks hyperuricemia followed by 24 hours renal I/R.
All groups including sham control group were injected with the vehicle in which oxonic acid was dissolved (Distilled water). Also, sham control group and group II, were subjected for exposure of the renal pedicles in both side, but they were not subjected to any renal I/R.
Body weight and kidney weight were measured, also blood pressure was measured.
Blood samples were collected for biochemical estimations of:
1. Plasma Urea, creatinine and Uric acid level.
2. Plasma electrolyte level: Na+ and k+.
3. Pro-oxidant parameters: plasma and renal tissue Malondialdhyde (MDA).
4. Anti-oxidant parameters: plasma total anti-oxidant capacity.
5. Plasma and renal tissue levels of NO.
The present study revealed that:
In oxonic acid treated rats (group II)There weresignificant increase inplasma level of uric acid and plasma total antioxidant capacity compared to control rats.Also there were significant increase in renal tissue MDA,plasma nitrite level compared to control rats and a higher though statistically non- significant in renal tissue nitritecompared to control rats.On the other hand there were nonsignificant changes in plasma MDA leveland blood pressure as well as no changes in plasma levels of potassium and sodium.
In ischemia reperfusion rats (group III)significant increase in plasma level of urea,creatinine ,plasma and renal tissue levels of MDA were observed compared to control rats.Plasma level of total antioxidant capacitywas decreased significantlycompared to both control rats and oxonic treated group.There was a significant decrease in renal tissue nitrite compared to both controls and hyperuricemic group but no significant change in plasma nitrite.Although there were significant hyponatremia compared to control rats there were no changes in plasma levels of potassium.also blood pressure values showed no significant changes compared to its corresponding values in control rats.
This work was performed on adultmale Wistar rats, dividedinto 4 groups:
Group I:Sham control rats.
Group II: Hyperurecemic rats. Hyperurecemia was induced subcutaneous injection ofOxonic acid (2%) in a dose of 100 mg /Kg/day for 6 days for two weeks.
Group III: Renal Ischemic- reperfused rats (renal I/R).
Group IV: 2 weeks hyperuricemia followed by 24 hours renal I/R.
All groups including sham control group were injected with the vehicle in which oxonic acid was dissolved (Distilled water). Also, sham control group and group II, were subjected for exposure of the renal pedicles in both side, but they were not subjected to any renal I/R.
Body weight and kidney weight were measured, also blood pressure was measured.
Blood samples were collected for biochemical estimations of:
1. Plasma Urea, creatinine and Uric acid level.
2. Plasma electrolyte level: Na+ and k+.
3. Pro-oxidant parameters: plasma and renal tissue Malondialdhyde (MDA).
4. Anti-oxidant parameters: plasma total anti-oxidant capacity.
5. Plasma and renal tissue levels of NO.
The present study revealed that:
In oxonic acid treated rats (group II)There weresignificant increase inplasma level of uric acid and plasma total antioxidant capacity compared to control rats.Also there were significant increase in renal tissue MDA,plasma nitrite level compared to control rats and a higher though statistically non- significant in renal tissue nitritecompared to control rats.On the other hand there were nonsignificant changes in plasma MDA leveland blood pressure as well as no changes in plasma levels of potassium and sodium.
In ischemia reperfusion rats (group III)significant increase in plasma level of urea,creatinine ,plasma and renal tissue levels of MDA were observed compared to control rats.Plasma level of total antioxidant capacitywas decreased significantlycompared to both control rats and oxonic treated group.There was a significant decrease in renal tissue nitrite compared to both controls and hyperuricemic group but no significant change in plasma nitrite.Although there were significant hyponatremia compared to control rats there were no changes in plasma levels of potassium.also blood pressure values showed no significant changes compared to its corresponding values in control rats.
Other data
| Title | Study on the Effects of Pre-existing Hyperuricemia and its Possible Pro-oxidant and Antioxidant Role on Renal Ischemia-reperfusion Injury in Male Albino Rats | Other Titles | دراسة تأثيرات الزيادة المبدئية بحامض البوليك واحتمالية دوره المؤكسد والمضاد للأكسدة على إصابة الكلى نتيجة قطع وإعادة التروية فى الفئران الذكور | Authors | Elsayed Ghonamy Mahros Ismail | Issue Date | 2016 |
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