In vitro studies on antitumor bioactive substances of Ecballium elaterium

Abstract

As mentioend in perviously in the objectives and workflow, the present study targeted establishment of tissue cultures from differet explants of Ecballium elaterium endmic in Egypt, optimization of factors affecting in vitro production of cucurbitacin E and I on shake flask and bioreactor levels and evaulation of potential activities of in vitro produced cucurbitacins as antimicrobial and antitumors. The most impotmat results (outcomes) of the study are as follows:

-The frequencies of callus formation differed significantly among different combinations of growth regulators and different explants. MS medium supplemented with 0.1 mg/1 NAA + 5 mg/1 BA (M5) showed the highest mean of callus formation then MS media supplemented with 5 mgll NAA + 0.1 mg/1 BA (M2). The maximum of callus percentage was observed with stem followed by root and leaf explants, respectively. MS medium supplemented with 5 mg/1 NAA + 0.1 mg/1 BA (M2) produced the highest callus fresh and dry weights

followed by MS medium supplemented with 0.1 mgll NAA + 5 mg/1 BA (M5)

and the stem explants produced the highest callus fresh and dry weights.