PROPAGATION OF SOME ORNAMENTAL PLANTS by TISSUE CULTURE
NASHWA ABD EL KADER ABD EL KADER;
Abstract
This investigation was carried out during the period from 2009 to 2012 at Ornamental Horticultur Department Fac. Of Agric. Cairo Univ. Giza Egypt. The experimental trial was conducted in Plant Tissue Culture Laboratory at El-Zohria Garden, Horticulture Research Institute, Agriculture Research Center, Ministry of Agriculture.
The aim of this study was to reach a well defined protocol protocol for in vitro propagation of Gardenia jasminoides Ellis, Cordline fruticosa Atom and Spathiphyllum mauna Loa. For, G. jasminoides, the best survival rate (100%) was found by using 1.5 % NaOCl for 25 min with zero% contamination and mortality. NAA at 4.0 mg/l during establishment stage, resulted in the highest shoot length and the highest number of leaves. For multiplication stage, 5mg/l BA was produced the highest number of shoots. At rooting stage, 3.0 mg/l IBA plus activated charcoal resulted the highest shootlet length and number of leaves. While, MS medium with activated charcoal contained 5.0 mg/l IBA produced the greatest number and length of roots. All plantlets acclimatized at pots containing 1:2 (v/v) peatmoss and sand. As for.Cordyline fruticosa, the highest survival percentage (100%) and the lowest mortality and contamination percentages were found at 2.0 % NaOCl for 25 min. At establishment stage, the highest number of shoots, shoot length and number of leaves were at full salt-strength of MS medium free hormon. While, callus formation were best at 5.0 mg/l NAA at different salt-strength of MS medium. For multiplication stage, the highest number of shoots resulted at 1.0 mg/l BA and 2.0 mg/l Kin. The highest shoot length and number of leaves were measured at control. The greatest callus formation was obtained at 4.0 mg/l BA and 3.0 mg/l kin. In the rooting stage, the highest shoot length, number of leaves, number of roots and root length were obtained on 3.0 mg/l IBA plus activated charcoal. For acclimatization stage, the highest plantlets length and number of leaves was achieved at pots containing 1:1(v/v) sand and peatmoss. Concerning. Spathiphyllum mauna, NaOCl at 2.0 % for 30 min resulted the largest survival percentage (100%) and lowest mortality or contamination percentage (0.0 %). at establishment stage, the highest number of shoots, number of leaves and shoot length was obtained on MS medium at full salt stregth plus 3.0 mg/l NAA. In the multiplication stage, 4.0 mg/l BA and 3.0 mg/l Kin was gave the highest number of shoots. Control treatment resulted the highest shoot length and number of leaves. Rooting medium plus 3.0 mg/l IBA without activated charcoal was the most effective to shoot length, number of leaves,but the best number of roots was obtained on MS medium plus1.0 mg/l IBA without activated charcoal., the highest root length was achieved on MS medium containing3.0 mg/l IBA plus activated charcoal. A mixture of peatmoss and sand at 1:2 (v/v) gave the highest number of leaves and plantlets length at acclimatization stage.
Key words: Micropropagation, Tissue Culture, in vitro, callus, Gardenia jasminoides,
Cordyline fruticosa, Spathiphyllum mauna.
The aim of this study was to reach a well defined protocol protocol for in vitro propagation of Gardenia jasminoides Ellis, Cordline fruticosa Atom and Spathiphyllum mauna Loa. For, G. jasminoides, the best survival rate (100%) was found by using 1.5 % NaOCl for 25 min with zero% contamination and mortality. NAA at 4.0 mg/l during establishment stage, resulted in the highest shoot length and the highest number of leaves. For multiplication stage, 5mg/l BA was produced the highest number of shoots. At rooting stage, 3.0 mg/l IBA plus activated charcoal resulted the highest shootlet length and number of leaves. While, MS medium with activated charcoal contained 5.0 mg/l IBA produced the greatest number and length of roots. All plantlets acclimatized at pots containing 1:2 (v/v) peatmoss and sand. As for.Cordyline fruticosa, the highest survival percentage (100%) and the lowest mortality and contamination percentages were found at 2.0 % NaOCl for 25 min. At establishment stage, the highest number of shoots, shoot length and number of leaves were at full salt-strength of MS medium free hormon. While, callus formation were best at 5.0 mg/l NAA at different salt-strength of MS medium. For multiplication stage, the highest number of shoots resulted at 1.0 mg/l BA and 2.0 mg/l Kin. The highest shoot length and number of leaves were measured at control. The greatest callus formation was obtained at 4.0 mg/l BA and 3.0 mg/l kin. In the rooting stage, the highest shoot length, number of leaves, number of roots and root length were obtained on 3.0 mg/l IBA plus activated charcoal. For acclimatization stage, the highest plantlets length and number of leaves was achieved at pots containing 1:1(v/v) sand and peatmoss. Concerning. Spathiphyllum mauna, NaOCl at 2.0 % for 30 min resulted the largest survival percentage (100%) and lowest mortality or contamination percentage (0.0 %). at establishment stage, the highest number of shoots, number of leaves and shoot length was obtained on MS medium at full salt stregth plus 3.0 mg/l NAA. In the multiplication stage, 4.0 mg/l BA and 3.0 mg/l Kin was gave the highest number of shoots. Control treatment resulted the highest shoot length and number of leaves. Rooting medium plus 3.0 mg/l IBA without activated charcoal was the most effective to shoot length, number of leaves,but the best number of roots was obtained on MS medium plus1.0 mg/l IBA without activated charcoal., the highest root length was achieved on MS medium containing3.0 mg/l IBA plus activated charcoal. A mixture of peatmoss and sand at 1:2 (v/v) gave the highest number of leaves and plantlets length at acclimatization stage.
Key words: Micropropagation, Tissue Culture, in vitro, callus, Gardenia jasminoides,
Cordyline fruticosa, Spathiphyllum mauna.
Other data
| Title | PROPAGATION OF SOME ORNAMENTAL PLANTS by TISSUE CULTURE | Other Titles | اكثار بعض نباتات الزينة بطريقة مزراع الانسجة. | Authors | NASHWA ABD EL KADER ABD EL KADER | Issue Date | 2014 |
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