Utility of Variable Analytical Methods for Micro Determination of Some Antidepressant

Abstract

This thesis contains three main parts. The first part includes a simple and sensitive spectrophotometric method for determination of Tramadol hydrochloride, Mianserin hydrochloride, Fluoxetine hydrochloride, Clomipramine hydrochloride and Dothiepin (Dosulepin) hydrochloride through ion-associates formation with phenol red (PhR), cresol red (CR), bromocresol green (BCG), metanil yellow (MY), methyl orange (MO) and bromothymol blue (BTB). The absorbance of each ion-associate was measured at maximum wavelength. Different factors were studied as pH, temperature and interfering ions to establish the best conditions for the determination. Comparison of the results of the proposed methods with those of official ones and complete validation study were also performed. The second part is based on thin layer chromatography methods for the detection of the above drugs. This method is analytical technique for the separation and identification of drugs due to its simplicity (it requires a less sophisticated apparatus), low cost, and need for minimal sample cleanup.