Biochemical Studies on Some Enzymes Used in Industry
Samia Abdel-Aziz Ahmed;
Abstract
The present investigation deals with physiological and biochemical studies on bacterial milk-clotting enzyme. It includes studies aiming at searching for a potent bacterial strain producing active milk-clotting enzyme.
The studies also included the production of the enzyme in batch and continuous cultures, by the free and immobilized cells of B. circulans25.
The studies also include the immobilization of enzyme on different carries. Separation, fractionation, purification, and properties of the purified enzyme were also investigated.
Bacillus circulans 25 (at 24 h) showed the highest ratio of MCA/ caseinase activity
(5.9).
The effect of some cultural condition (carbon basis, nitrogen sources, KH2P04
concentration,...etc) on the productivity of MCE by B. circulans 25 was investigated.
Different matrices were investigated for the production of MCE by the immobilized cells of B. circulans 25. Of all preparations, the immobilized cells of B. circulans 25 on wool (after 24h), showed the highest MCA (1.0 U/ml with MCA/caseinse activity ratio of 11.11) and the highest specific productivity (5.2 U/g wet cells/h).
The studies also included the production of the enzyme in batch and continuous cultures, by the free and immobilized cells of B. circulans25.
The studies also include the immobilization of enzyme on different carries. Separation, fractionation, purification, and properties of the purified enzyme were also investigated.
Bacillus circulans 25 (at 24 h) showed the highest ratio of MCA/ caseinase activity
(5.9).
The effect of some cultural condition (carbon basis, nitrogen sources, KH2P04
concentration,...etc) on the productivity of MCE by B. circulans 25 was investigated.
Different matrices were investigated for the production of MCE by the immobilized cells of B. circulans 25. Of all preparations, the immobilized cells of B. circulans 25 on wool (after 24h), showed the highest MCA (1.0 U/ml with MCA/caseinse activity ratio of 11.11) and the highest specific productivity (5.2 U/g wet cells/h).
Other data
| Title | Biochemical Studies on Some Enzymes Used in Industry | Other Titles | دراسات كيميائية حيوية على بعض الانزيمات المستخدمة فى الصناعة | Authors | Samia Abdel-Aziz Ahmed | Issue Date | 2003 |
Attached Files
| File | Size | Format | |
|---|---|---|---|
| سامية عبد العزيز.pdf | 238.53 kB | Adobe PDF | View/Open |
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