ROLE OF MELATONIN ON INDUCED RADIATION EFFECTS DURING CANCER TREATMENT
SHiMAA FARAG HEMIDA HUSSIEN;
Abstract
Cancer may affect people at all ages. It can be treated and some cured, depending on the specific type, location, and stage. Cancer is usually treated with a combination of surgery and radiotherapy. Radiation therapy works by damaging the DNA of cells. The damage is caused by a photon, electron, proton, neutron, or ion beam directly or indirectly ionizing the atoms which make up the DNA chain. Indirect ionization happens as a result ofthe ionization ofwater, forming free radicals, notably hydroxyl radicals, which then damage the DNA, besides DNA, lipids and proteins are also attacked by free radical. Thus drug or antioxidant that scavenge or inhibit the formation of these radicals may have relevance to cancer patients by ameliorating damage of normal tissue exposed to ionizing irradiation therapy.
The present study is concerned with the changes in the biophysical and biochemical properties the RBCs because of the possibility to isolate each RBC from the other for the study of their membrane properties and hemoglobin molecular structure a process which is not possible for other cells from the different organs.
The results of Hemoglobin absorption indicate that the average value of, peak position, width at half maximum Whmax of sort band and ratio between the met hemoglobin to oxy-hemoglobin A578 I A540 had been altered by irradiation either whole body or local body irradiation. A pronounced increase in Whmax followed by a decrease in the absorbance of sort band which reflects degradation of hemoglobin molecular structure and decrease absorption ratio A578 I A540 in addition to the disappearance of the globin band at 275 nm were detected by injection of animals with cancer cells or exposing to y-irradiation or both as compared to the control animals. This alteration is considered to be an evidence for the free radicals formation by y-irradiation which induced deteriorating effect on the antioxidant system. This promoted oxy hemoglobin to met hemoglobin. Treating animals with melatonin before tumor cells injection or exposing to radiation some sort of protection occurs as compared to normal hemoglobin.
Electron spin resonance spectroscopy (ESR) is considered to be a sensitive tool to detect free radicals which expected to be formed by irradiation. The formation of ESR
The present study is concerned with the changes in the biophysical and biochemical properties the RBCs because of the possibility to isolate each RBC from the other for the study of their membrane properties and hemoglobin molecular structure a process which is not possible for other cells from the different organs.
The results of Hemoglobin absorption indicate that the average value of, peak position, width at half maximum Whmax of sort band and ratio between the met hemoglobin to oxy-hemoglobin A578 I A540 had been altered by irradiation either whole body or local body irradiation. A pronounced increase in Whmax followed by a decrease in the absorbance of sort band which reflects degradation of hemoglobin molecular structure and decrease absorption ratio A578 I A540 in addition to the disappearance of the globin band at 275 nm were detected by injection of animals with cancer cells or exposing to y-irradiation or both as compared to the control animals. This alteration is considered to be an evidence for the free radicals formation by y-irradiation which induced deteriorating effect on the antioxidant system. This promoted oxy hemoglobin to met hemoglobin. Treating animals with melatonin before tumor cells injection or exposing to radiation some sort of protection occurs as compared to normal hemoglobin.
Electron spin resonance spectroscopy (ESR) is considered to be a sensitive tool to detect free radicals which expected to be formed by irradiation. The formation of ESR
Other data
| Title | ROLE OF MELATONIN ON INDUCED RADIATION EFFECTS DURING CANCER TREATMENT | Other Titles | دور الميلاتونين فى تقليل الأثار الجانبية للإشعاع أثناء علاج الأورام السرطانية | Authors | SHiMAA FARAG HEMIDA HUSSIEN | Issue Date | 2009 |
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