Cytogenetic Effects of Some Stored Seeds Protectants Against Bruchids Pest on Vicia faba Plant

Abstract

The present work deals with the cytogenetic effects of four stored seeds protectants against Bruchids pests (Callosobruchus maculates F and C. chinensis L.) on Vicia faba plant. The studies includes the effect of: i) dusting the seeds with different concentrations of “Malathion 1% powder” (Mal.) and fumigation of other with “Phosphine” (Phos.) for storage as synthetic insecticides active against Bruchids pest and recommended to be used in Egypt for protection of beans and grains, since1980 till now and (ii) dusting the seeds with different concentrations of either "Lupinus termis seeds powder" (LtSP) or black cumin seeds powder (bcSP) for storage as two plant products proved to be active against the same target pest. This study aims to evaluate the effect of the four agents after six months storage of the treated seeds on the: mitotic and meiotic apparatuses, some metric traits of the F1 &F2 plants and changes on protein profiles of M2 seeds; In addition, changes in some cytoplasmic organelles induced in the cells of Vicia faba root-tip meristems after treatment, took place by the use of TEM observations.